Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Introduction: Cold static storage (CSS) and normothermic ex-situ preservation are the most widely used donor heart preservation techniques worldwide. The current study compares both CSS and normothermic ex-situ preservation methods in terms of graft performance, morphologic changes, and acute immune response in an experimental model.
Method And Materials: Twenty rats underwent heterotopic abdominal heart transplantation after 2 h of CSS (group 1; n = 10) or normothermic ex-situ perfusion (group 2; n = 10). Blood samples were obtained from recipients just before and after 4 h of transplantation to analyze surface markers of immune cells and cytokines. Electrocardiography and echocardiography were performed before donor heart harvesting and after heterotopic transplantation. After 4 h of transplantation, donor hearts were extracted for further histologic studies.
Results: All recipient animals in both groups successfully survived after heterotopic transplantation. The mean ischemic time of the donor heart was 163 ± 8.34 mins in group 1 and 43.8 ± 6.97 mins in group 2 (P < 0.01). Ejection fraction significantly decreased after transplantation in both groups but were less significant in group 2 (the mean difference group 1: -34.3 ± 3.54, P < 0.01; group 2: -14.3 ± 15.47, P = 0.01). The percentage of granulocyte significantly increased in both group 1 and group 2, but the significance was more pronounced in group 1 (the mean difference group 1: 48.7 ± 5.36, P < 0.01; group 2: 39.7 ± 13.1, P < 0.01).
Conclusions: Normothermic ex-situ perfusion is associated with well-preserved donor hearts but a similar recipient acute immune response in comparison with CSS in the rat model.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jss.2024.12.050 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
From critical care to organ preservation: the potential role of hemadsorption in transplantation-a narrative review.
Hepatobiliary Surg Nutr
August 2025
Department of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA.
Background And Objective: Hemadsorption, a therapeutic modality traditionally used to mitigate cytokine storm in critically ill patients and is now being tested in other medical fields including organ transplantation, especially for organs from extended criteria donors. Such organs are susceptible to ischemia-reperfusion injury (IRI) during transplantation, which is known to release a cascade of innate immune mediators including damage-associated molecular patterns (DAMPs) and cytokines during reperfusion and ultimately leading to graft dysfunction based on the metabolic injury. To address this challenge, early studies have integrated hemadsorption filters into organ perfusion circuits, such as normothermic regional perfusion (NRP) in donors and machine perfusion (MP) of grafts, with the aim to reduce inflammation and improve organ viability.
View Article and Find Full Text PDFTransplantation of Severely Steatotic Liver Grafts After Machine Perfusion Remains a Risky Challenge.
Clin Transplant
August 2025
Department of Surgery, Division of HPB Surgery and Liver transplantation, University of Groningen and University Medical Center Groningen, Groningen, the Netherlands.
Liver transplantation is the treatment of choice for patients with end-stage liver disease. However, donor shortages have increased the use of high-risk and extended criteria donor livers, including livers donated after circulatory death and those with severe steatosis. Severe donor liver steatosis is associated with poor outcomes due to high susceptibility to ischemia-reperfusion injury.
View Article and Find Full Text PDFOptimizing liver utilization for transplantation with partial grafts undergoing normothermic machine perfusion: Two case reports.
World J Transplant
September 2025
Division of Abdominal Transplantation, Department of Surgery, Carolinas Medical Center, Charlotte, NC 28203, United States.
Background: Liver transplantation (LT) is the only curative, life-saving option for children and adults with end-stage liver disease. Due to the well-known shortage and heterogeneity of grafts, split LT (SLT) is an attractive strategy to expand the donor pool and reduce waitlist times. Given increased risk of cold ischemia time with SLT, machine perfusion represents a promising option to reduce it and optimize transplant logistics and outcomes.
View Article and Find Full Text PDFThe Hitchhiker's guide to isolated organ perfusion: a journey to 2040.
Front Transplant
August 2025
The Nuffield Department of Surgical Sciences, University of Oxford, Oxford, United Kingdom.
Building on the established success of hypothermic machine perfusion (HMP) and emerging normothermic platforms, machine perfusion is poised to guide a journey toward 2040, transforming organ transplantation into an era of integrated preservation, viability assessment, and ex situ therapy. While renal HMP today reduces delayed graft function and improves graft survival, the next two decades will centre on adaptive platform trials in normothermic perfusion, predictive AI-driven biomarkers, and unified registries to validate robust surrogate endpoints. Centralised Assessment and Reconditioning Centres (ARCs) will streamline 24/7 workflows, combining advanced imaging, molecular assays, and gene or cell therapies to repair and optimise grafts ex-vivo.
View Article and Find Full Text PDFMyocardial Work Index as an Indicator of Cardiac Function in Ex Situ Heart Perfusion.
J Heart Lung Transplant
August 2025
Department of Cardiac Surgery and Transplantation, Marie Lannelongue Hospital, Groupe Hospitalier Paris Saint Joseph, Le Plessis Robinson, France; Inserm UMR-S 999, School of Medicine, University of Paris Saclay, Le Kremlin Bicêtre, France.
Heart transplantation is increasingly reliant on marginal grafts due to organ shortages. Ex situ heart perfusion (ESHP) enables viability assessment, but current methods lack functional evaluation. We aimed to investigate the relationship between echocardiographic myocardial work index (MWI) and cardiac performance during ESHP.
View Article and Find Full Text PDF