Deep Eutectic Solvent-Assisted Synthesis of Ni-Graphene Composite Supported on Screen-Printed Electrodes for Biogenic Amine Detection.

Deep eutectic solvents (DES) have emerged as versatile, sustainable media for the synthesis of nanomaterials due to their low toxicity, tunability, and biocompatibility. This study develops a one-step method to modify commercially available screen-printed electrodes (SPE) using laser-induced pyrolysis of DES, consisting of choline chloride and tartaric acid with dissolved nickel acetate and dispersed graphene. The electrodes were patterned using a 532 nm continuous-wave laser for the in situ formation of Ni nanoparticles decorated on graphene sheets directly on the SPE surface (Ni-G/SPE). The synthesis parameters, specifically laser power and graphene concentration, were optimized using the Nelder-Mead method to produce modified Ni-G/SPEs with maximized electrochemical response to dopamine. Electrochemical characterization of the developed sensor by differential pulse voltammetry revealed its broad linear detection range from 0.25 to 100 μM and high sensitivity with a low detection limit of 0.095 μM. These results highlight the potential of laser-assisted DES synthesis to advance electrochemical sensing technologies, particularly for the detection of biogenic amines.