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Background: Postharvest lemons are affected by several fungal infections, and as alternatives to chemical fungicides for combating these infections, different microbial biocontrol agents have been studied, with the Clavispora lusitaniae 146 strain standing out. Although strain 146 has proven to be an effective agent, the influence of a microbial biological control agent on the postharvest lemon microbiome has not been studied until now. Thus, this study aimed to evaluate how the epiphytic microbiome of postharvest lemons is affected by the application of the biocontrol yeast C. lusitaniae 146.
Results: In terms of bacterial composition, the most abundant genera were Sphingomonas, Pelomonas, and Bacillus and no significant differences in the composition were detected between the treated and control samples. Among fungi, Clavispora was predominant not only in the treated samples but also in the control, and statistics indicated differences, suggesting its significant role in modulating the epiphytic community composition of lemon. Understanding fruit microbiomes is vital for effective disease control, and this study provides insights into the microbial composition of the surface of lemon and the role of C. lusitaniae 146.
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http://dx.doi.org/10.1186/s13104-024-07064-4 | DOI Listing |
Pest Manag Sci
July 2025
Pilot Plant of Industrial Microbiological Processes (PROIMI)-National Scientific and Technical Research Council (CONICET), Tucumán, Argentina.
Background: The worldwide citrus industry faces significant economic losses as a consequence of fungal infections, primarily green mold caused by Penicillium digitatum. In Argentina, a leading lemon producer, these losses pose a substantial challenge to domestic and international markets. Although synthetic fungicides are widely used for green mold control, their effectiveness is hampered by pathogen resistance and growing concerns regarding environmental contamination from chemical residues.
View Article and Find Full Text PDFBMC Res Notes
January 2025
Planta Piloto de Procesos Industriales Microbiológicos (PROIMI - CONICET), Tucumán, Argentina.
Background: Postharvest lemons are affected by several fungal infections, and as alternatives to chemical fungicides for combating these infections, different microbial biocontrol agents have been studied, with the Clavispora lusitaniae 146 strain standing out. Although strain 146 has proven to be an effective agent, the influence of a microbial biological control agent on the postharvest lemon microbiome has not been studied until now. Thus, this study aimed to evaluate how the epiphytic microbiome of postharvest lemons is affected by the application of the biocontrol yeast C.
View Article and Find Full Text PDFMicroorganisms
September 2020
Planta Piloto de Procesos Industriales Microbiológicos (PROIMI) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Belgrano y Pje. Caseros, 4000 Tucumán, Argentina.
Fungal rots are one of the main causes of large economic losses and deterioration in the quality and nutrient composition of fruits during the postharvest stage. The yeast 146 has previously been shown to efficiently protect lemons from green mold caused by . In this work, the effect of yeast concentration and exposure time on biocontrol efficiency was assessed; the protection of various citrus fruits against by 146 was evaluated; the ability of strain 146 to degrade mycotoxin patulin was tested; and the effect of the treatment on the sensory properties of fruits was determined.
View Article and Find Full Text PDFPLoS One
November 2020
Planta Piloto de Procesos Industriales Microbiológicos (PROIMI-CONICET), Tucumán, Argentina.
Only quite recently, we have shown that yeast strains Clavispora lusitaniae 146 and Pichia fermentans 27 can act as efficient biocontrol agents for combating postharvest fungal diseases in lemons. During postharvest and storage conditions, microorganisms are subject to different stress factors that could affect both their survival and their protective capacity. Understanding the tolerance of yeasts to environmental stress factors could support the future development and commercial application of biological control formulations based on such organisms.
View Article and Find Full Text PDFInt J Food Microbiol
February 2018
Westerdijk Fungal Biodiversity Institute, Uppsalalaan 8, 3584 Utrecht, The Netherlands. Electronic address:
Since food spoilage by yeasts causes high economic losses, fast and accurate identifications of yeasts associated with food and food-related products are important for the food industry. In this study the efficiency of the matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) to identify food related yeasts was evaluated. A CBS in-house MALDI-TOF MS database was created and later challenged with a blinded test set of 146 yeast strains obtained from food and food related products.
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