Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Background: The contraction behaviors of cardiomyocytes (CMs), especially contraction synchrony, are crucial factors reflecting their maturity and response to drugs. A wider field of view helps to observe more pronounced synchrony differences, but the accompanied greater computational load, requiring more computing power or longer computational time.
Methods: We proposed a method that directly correlates variations in optical field brightness with cardiac tissue contraction status (CVB method), based on principles from physics and photometry, for rapid video analysis in wide field of view to obtain contraction parameters, such as period and contraction propagation direction and speed.
Results: Through video analysis of human induced pluripotent stem cell (hiPSC)-derived CMs labeled with green fluorescent protein (GFP) cultured on aligned and random nanofiber scaffolds, the CVB method was demonstrated to obtain contraction parameters and quantify the direction and speed of contraction within regions of interest (ROIs) in wide field of view. The CVB method required less computation time compared to one of the contour tracking methods, the Lucas-Kanade (LK) optical flow method, and provided better stability and accuracy in the results.
Conclusion: This method has a smaller computational load, is less affected by motion blur and out-of-focus conditions, and provides a potential tool for accurate and rapid analysis of cardiac tissue contraction synchrony in wide field of view without the need for more powerful hardware.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11794902 | PMC |
| http://dx.doi.org/10.1007/s13770-024-00688-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Use of telemedicine to provide initial home parenteral nutrition training during COVID-19 pandemic: A case series.
Nutr Health
September 2025
Division of General Internal Medicine, Mayo Clinic, Rochester, MN, USA.
BackgroundCoronavirus Disease 2019 (COVID-19) has led to dramatic changes including social distancing, closure of schools, travel bans, and issues of stay-at-home orders. The health-care field has been transformed with elective procedures and on-site visits being deferred. Telemedicine has emerged as a novel mechanism to continue to provide care.
View Article and Find Full Text PDFMinimally invasive and standardized thoracoscopic surgery for stage III empyema using a variable-view rigid endoscope.
Gen Thorac Cardiovasc Surg
September 2025
Department of General Thoracic Surgery, Seirei Hamamatsu General Hospital, 2-12-12, Hamamatsu, Shizuoka, 430-8558, Japan.
Thoracoscopic surgery for stage III acute empyema is often limited by poor visualization and anatomical complexity. We developed a standardized, minimally invasive approach using a variable-view rigid endoscope and fixed port placement, regardless of disease extent or patient physique. The variable-view endoscope enabled a wide, adjustable field of view without moving the camera shaft, allowing safe access even in the confined thoracic space.
View Article and Find Full Text PDFEfficient production of sodium Bose-Einstein condensates in a hybrid trap.
Rev Sci Instrum
September 2025
Joint Quantum Institute, University of Maryland and National Institute of Standards and Technology, College Park, Maryland 20742, USA.
We describe an apparatus that efficiently produces 23Na Bose-Einstein condensates (BECs) in a hybrid trap that combines a quadrupole magnetic field with a far-detuned optical dipole trap. Using a Bayesian optimization framework, we systematically optimize all BEC production parameters in modest-sized batches of highly correlated parameters. Furthermore, we introduce a Lagrange multiplier-based technique to optimize the duration of different evaporation stages constrained to have a fixed total duration; this enables the progressive creation of increasingly rapid experimental sequences that still generate high-quality BECs.
View Article and Find Full Text PDFPopulation-level bistability in quorum sensing.
mBio
September 2025
Department of Microbiology, Oregon State University, Corvallis, Oregon, USA.
Quorum sensing (QS) is a widespread signaling mechanism in bacteria that coordinates collective behaviors according to population density. A foundational assumption in this field is that QS functions as a gene expression switch that synchronizes responses at the population level. While some studies indeed report homogeneous on/off transitions, others report heterogeneity at the cellular level, challenging the canonical view.
View Article and Find Full Text PDFHigh-resolution imaging system for integration into intelligent noncontact total body scanner.
J Biomed Opt
September 2025
Leibniz University Hannover, Hannover Centre for Optical Technologies, Hannover, Germany.
Significance: Melanoma's rising incidence demands automatable high-throughput approaches for early detection such as total body scanners, integrated with computer-aided diagnosis. High-quality input data is necessary to improve diagnostic accuracy and reliability.
Aim: This work aims to develop a high-resolution optical skin imaging module and the software for acquiring and processing raw image data into high-resolution dermoscopic images using a focus stacking approach.