Simultaneous degradation of roxithromycin and nitrogen removal by Acinetobacter pittii TR1: Performances, pathways, and mechanisms.

Pharmaceutical and aquaculture wastewater contains not only antibiotics but also high concentrations of nitrogen, but few studies have been conducted on bacteria that target this complex pollution for degradation. A novel heterotrophic nitrifying aerobic denitrifying (HN-AD) strain Acinetobacter pittii TR1 isolated from soil. When the C/N ratio was 20, the strain could degrade 50 mg/L roxithromycin (ROX) and the nitrogen removal rate was 96.06% with no accumulation of nitrite. The nitrogen metabolism pathway of strain TR1 was conjectured by NH-N → (NHOH) → NO-N → NO-N → NO-N → (NO → NO → N). Nitrogen balance analysis showed that 50.43% of the initial total nitrogen (TN) was converted to gaseous nitrogen and 45.39% was assimilated by TR1. Lower concentrations of ROX affected the activity of key enzymes, and the expression of the denitrifying genes hao, napA, nirK, norZ, and nosZ were significantly up-regulated, with the gene expression of nirK being up-regulated by 15.9-fold. Cleavage of desosamine, demethylation, and phosphorylation were the main biotransformation pathways of ROX. This work offers fresh insights into the metabolic processes of HN-AD bacteria under antibiotic stress, as well as evidence supporting strain TR1 in the treatment of wastewater with nitrogen and ROX.