Investigation of mono-nuclear cobalt(II) complexes with a tri-dentate quinoxalyl-hydrazone ligand for their potential in biological research and interaction with ct-DNA.

The condensing reaction of 2-hydroxy-1-naphthaldehyde with quinoxalyl-2-carbohydrazide resulted in synthesizing of a novel derivative of hydrazone quinoxalyl ligand (Hdpq). The bonding behavior between Hdpq and Co(II) ion was investigated in molar ratios of 1: 1 and 2: 1 to produce two different complexes, Codpq and Co(dpq), respectively. Their chemical structure was verified using several spectroscopic approaches. The characterization envolved micro-C, H, and N-elemental analyses, thermogravimetric investigations, as well as the examination of their magnetic and conductance properties. The inhibitory effects of Hdpq (the free ligand) and its Co(II)-chelates on the growing up of three specific bacterial series, three specific fungal series, and three famous human cancer cell lines were evaluated based on their structure features. The study referred to the pivotal function of Co(II) and its ratio in the two complexes. The antioxidant activity was determined for all current compounds within DPPH and SOD assays, reporting respectable antioxidant reactivity. The interaction of Hdpq, Codpq, and Co(dpq) with calf thymus DNA (ct-DNA) was assessed by analyzing through the alterations in the viscometric and spectrophotometric properties. Determination of binding constant (K, 13.12, 16.02, and 16.82 × 10 mol dm), Gibb's free energy (∆G, were -40.21, -46.13, and -46.67 kJ mol), and the chromism mode (hypo-character) were employed for Hdpq, Codpq, and Co(dpq) to assess the interactive modes, respectively. Rewardingly, the monometallic-chelates Co(II)-complexes displayed modified binding action more than that of Hdpq against ct-DNA.