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Article Abstract
Objective: To investigate the molecular mechanism and distribution characteristics of RhD negative phenotypes in Han population of blood donors in Wuhu city.
Methods: A total of 210 RhD samples from August 2021 to August 2022 were screened by serological test and collected from Wuhu Central Blood Station for the voluntary blood donor population. Exons 1 and 10 of the gene were amplificated by PCR to determine whether the samples had the gene. Exons 1-10 of the gene were amplificated by PCR and zygosity analysis were performed in 82 samples containing D gene, and Sanger sequencing was performed on 55 samples containing all exons to determine the genotype.
Results: Among 210 RhD specimens, 128 cases (60.38%) had gene deletion. 27 cases had partial exons of , including 2 cases with , 24 cases with , and 1 case with . 55 cases had retained all of 10 exons, including 4 cases with , 6 cases with , 1 case with , 1 case with , 39 cases with , and the remaining 4 cases were determined to have no gene deletion by zygosity analysis and sequencing showed the presence of 1227G>A mutation loci.
Conclusion: There is polymorphism in the molecular mechanism of RhD D gene in Wuhu blood donor population, among which are the main variants in this region. The results of this study provide a theoretical basis for RhD blood group identification and clinical blood transfusion in this region.
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| http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2024.05.035 | DOI Listing |
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