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http://dx.doi.org/10.1111/cea.14585 | DOI Listing |
iScience
August 2025
Department of Gastroenterology, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
is a frequently mutated gene in gastric cancers (GCs), particularly in those associated with the Epstein-Barr virus (EBV), which also often shows mutations and silencing. However, the role of these alterations in the development of GC remains unclear. Here, using Cre; ; ; LSL- mice (APP mice), we found that deletion alone promoted a type 2 immune microenvironment marked by the infiltration of type 2 innate lymphoid cells (ILC2s), eosinophils, mast cells, and M2 macrophages via triggering aberrant IL-33-expressing pit lineage differentiation in stem/progenitor cells.
View Article and Find Full Text PDFJ Immunol Methods
March 2025
ICAR-Indian Veterinary Research Institute, Bangalore, Karnataka 560024, India.
Bluetongue (BT) is a vector-borne viral disease of multiple domestic and wild ruminants across the globe. The VP7 protein of bluetongue virus (BTV) is the major immune-dominant structural protein that is conserved across the BTV serotypes and therefore, targeted for the development of immuno-diagnostics for BT. In this study, full-length recombinant VP7 protein (rVP7) of BTV-1 was expressed in Trochoplusia ni derived insect cells (Tn5) using codon-optimized synthetic gene construct through baculovirus expression system.
View Article and Find Full Text PDFClin Exp Allergy
January 2025
Center for Translational Immunology, University Medical Center Utrecht, Utrecht, The Netherlands.
Cureus
May 2024
Department of Microbiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.
Introduction , designated as a priority pathogen by the World Health Organization (WHO), is responsible for recalcitrant infections in immunocompromised patients. The type VI secretion system (T6SS) is a class of macromolecular secretion machines, contributing to its virulence. The aim of this study is thus to predict the immune-dominant epitope peptides from the Acinetobacter T6SS-associated protein of (AsaA).
View Article and Find Full Text PDFMol Ther Methods Clin Dev
December 2023
Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA.
Current immunotherapeutic targets are often shared between neoplastic and normal hematopoietic stem and progenitor cells (HSPCs), leading to unwanted on-target, off-tumor toxicities. Deletion or modification of such targets to protect normal HSPCs is, therefore, of great interest. Although HSPC modifications commonly aim to mimic naturally occurring phenotypes, the long-term persistence and safety of gene-edited cells need to be evaluated.
View Article and Find Full Text PDF