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The sensory quality of a wine is mainly based on its aroma and flavor. Sweetness contributes in the gustatory balance of red wines. The investigation of compounds involved in this flavor was based on empirical observations, such as the increase in wine sweetness during yeast autolysis, concomitant to post-fermentation maceration in red winemaking. An untargeted metabolomics approach using UHPLC-HRMS has been developed to discover a new sweet molecule released during this stage. Among several markers highlighted, one compound was selected to be isolated by various separative techniques. It was unambiguously identified by NMR as N6-succinyladenosine and is reported for the first time in wine at an average concentration of 3.16 mg/L in 85 red wines. Furthermore, sensory analysis has highlighted its sweetness. In addition to discovering a new sweet compound in wine, this study proposes new tools for studying taste-active compounds in natural matrices.
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http://dx.doi.org/10.1016/j.foodchem.2024.140801 | DOI Listing |
J Neurooncol
September 2025
Department of Neurological Surgery, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
Purpose: NOTCH3 is increasingly implicated for its oncogenic role in many malignancies, including meningiomas. While prior work has linked NOTCH3 expression to higher-grade meningiomas and treatment resistance, the metabolic phenotype of NOTCH3 activation remains unexplored in meningioma.
Methods: We performed single-cell RNA sequencing on NOTCH3 + human meningioma cell lines.
Food Res Int
November 2025
Tea Research Institution, Yunnan Academy of Agricultural Sciences, Kunming 650200, China; Yunnan Key Laboratory of Tea Science, Tea Research Institution, Yunnan Academy of Agricultural Sciences, Kunming 650200, China. Electronic address:
Pu-erh raw tea (PRT), a post-fermented tea, is prized for its complex flavor profile and health-promoting properties. While extended storage enhances its sensory attributes, the decade-scale metabolic dynamics underpinning flavor evolution remain unexplored. This study comprehensively characterized non-volatile metabolomic profiles and flavor changes in PRT across a 10-year storage period (2012-2023).
View Article and Find Full Text PDFRapid Commun Mass Spectrom
September 2025
Département Médicaments et Technologies pour la Santé (DMTS), MetaboHUB, Université Paris-Saclay, CEA, INRAE, Gif sur Yvette, France.
Rationale: Electrospray (ESI), the most popular desorption/ionization technique used in mass spectrometry-based metabolomics, generates both protonated and deprotonated molecules, as well as adduct ions, sodium being the most frequent monoatomic cation entering their composition. With the spread and generalization of untargeted data-dependent and independent tandem mass spectrometry experiments, considering product ion spectra of sodium-containing entities appears relevant to complement fragmentation information of their protonated and deprotonated counterparts.
Methods: Solutions of pure standards, mainly amino and organic acids, were prepared at 1 μg/mL and injected either by direct infusion or by flow-injection prior to ESI-MS/MS analysis.
Biomed Chromatogr
October 2025
Department of Hepatobiliary Hydatid Surgery, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region, China.
Hepatic cystic echinococcosis (HCE), a liver manifestation of hydatid disease, is among the 17 neglected tropical diseases (NTDs) prioritized by the WHO for eradication by 2025. Although imaging and serological tests are currently the main diagnostic approaches for HCE, they have notable limitations in sensitivity and specificity. Here, we applied liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based metabolomic profiling to uncover differential metabolites and highlight disrupted metabolic pathways, aiming to identify candidate biomarkers for HCE diagnosis.
View Article and Find Full Text PDFPLoS One
September 2025
Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Animal Science and Technology, Foshan University, Foshan, China.
Double-Layer Steamed Milk Custard (DLSMC) is a famous traditional Chinese dessert. This study aimed to analyze the flavor and the changes in metabolites during different stages of DLSMC preparation, including raw buffalo milk, thermo-processing, first and second-layer milk skin formation. Electronic nose and electronic tongue were employed to preliminarily assess the overall flavor characteristics between these stages.
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