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http://dx.doi.org/10.1002/hem3.134 | DOI Listing |
Mol Ecol Resour
September 2025
Centre for Evolutionary Hologenomics (CEH), Globe Institute, University of Copenhagen, Copenhagen, Denmark.
Global efforts to standardise methodologies benefit greatly from open-source procedures that enable the generation of comparable data. Here, we present a modular, high-throughput nucleic acid extraction protocol standardised within the Earth Hologenome Initiative to generate both genomic and microbial metagenomic data from faecal samples of vertebrates. The procedure enables the purification of either RNA and DNA in separate fractions (DREX1) or as total nucleic acids (DREX2).
View Article and Find Full Text PDFPest Manag Sci
September 2025
AgResearch Ltd, Tuhiraki, Lincoln, New Zealand.
Background: Conventional weed risk assessments (WRAs) are time-consuming and often constrained by species-specific data gaps. We present a validated, algorithmic alternative, the model, that integrates climatic suitability ( ), weed-related publication frequency (P) and global occurrence data ( ), using publicly available databases and artificial intelligence (AI)-assisted text screening with a large language model (LLM).
Results: The model was tested against independent weed hazard classifications for New Zealand and California.
Environ Res
September 2025
Department of Environment and Energy, Sejong University, Seoul 05006, South Korea. Electronic address:
Identifying the sources of sedimentary organic matter (OM) is essential for understanding pollution dynamics and guiding effective management in estuarine environments. This study proposes a novel and transferable source tracking framework that integrates Fourier transform infrared (FTIR) and fluorescence spectroscopy with a principal component analysis-absolute principal component score-multiple linear regression (PCA-APCS-MLR) receptor model to apportion OM sources in surface sediments across four South Korean estuaries with contrasting land use. Five new infrared-based indices (IRIs), developed from diagnostic FTIR absorbance features of water-extractable organic matter (WEOM), were designed to capture source-specific functional group compositions linked to terrestrial, synthetic, and petroleum-derived OM.
View Article and Find Full Text PDFPLoS One
September 2025
Department of Molecular Biology and Genetics, Faculty of Science, Koç University, Istanbul, Türkiye.
The increasing demand for efficient recombinant insulin production necessitates the development of scalable, high-yield, and cost-effective bioprocesses. In this study, we engineered a novel mini-proinsulin (nMPI) with enhanced expression properties by shortening the C-peptide and incorporating specific residue substitutions to eliminate the need for enzymatic cleavage. To optimize its production, we applied a hybrid approach combining microscale high-throughput cultivation using the BioLector microbioreactor and statistical modeling via response surface methodology (RSM).
View Article and Find Full Text PDFAdv Mater
September 2025
KU-KIST Graduate School of Converging Science and Technology, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea.
Metal-nitrogen-carbon (M-N-C) catalysts display considerable potential as cost-effective alternatives to noble metals in oxygen electrocatalysis. However, uncontrolled atomic migration and random structural rearrangement during pyrolysis often lead to disordered coordination environments and sparse active sites, fundamentally limiting their intrinsic catalytic activities and long-term durability. Herein, a novel strategy is reported for use in directionally regulating atomic migration pathways via the incorporation of a foreign metal (La).
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