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The microbial enhanced oil recovery (MEOR) process has been identified as a promising alternative to conventional enhanced oil recovery methods because it is eco-friendly and economically advantageous. However, the knowledge about the composition and diversity of microbial communities in artificially regulated reservoirs, especially after activating petroleum hydrocarbon-degrading bacteria (PHDB) by injecting exogenous nutrients, is still insufficient. This study utilized a combination of high-throughput sequencing and metagenomics technology to reveal the structural evolution characteristics of the indigenous microbial community in the reservoir during the PHDB activated for enhanced oil recovery, as well as the response relationship between the expression of its oil production functional genes and crude oil biodegradation. Results showed that (>75%) gradually evolves into a stable dominant microbial community in the reservoir during the activation of PHDB. Besides, the gene expression and KEGG pathways after crude oil undergoes biodegradation by PHDB show that the number of genes related to petroleum hydrocarbon metabolism dominates the metabolism (21.98%). Meanwhile, a preliminary schematic diagram was drawn to illustrate the evolution mechanism of the EOR metabolic pathway after the targeted activation of PHDB. Additionally, it was found that the abundance of hydrocarbon-degrading enzymes increased significantly, and the activity of alcohol dehydrogenase was higher than that of aldehyde dehydrogenase and monooxygenase after PHDB activation. These research results not only filled in and expanded the theoretical knowledge of MEOR based on artificial interference or regulation of reservoir oil-recovery functional microbial community structure but also provided guidance for the future application of MEOR technology in oil field operations.
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http://dx.doi.org/10.1021/acsomega.3c10334 | DOI Listing |
Int J Orthop Trauma Nurs
August 2025
Autoimmune Diseases Research Center, Kashan University of Medical Sciences, Kashan, Iran. Electronic address:
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PLoS One
September 2025
Department of Environmental and Occupational Health and Safety, Institute of Public Health, College of Medicine and Other Health Sciences, University of Gondar, Gondar, Ethiopia.
Foodborne diseases pose a significant public health challenge worldwide. The increasing availability of edible oils in the market, combined with Ethiopia's lack of stringent quality control and regulatory oversight, raises concerns about their safety. This inadequacy in regulation may contribute to microbial contamination, leading to potential public health risks.
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Engineering Technology Research Center of Preparation and Application of Industrial Ceramics of Anhui Province, Engineering Research Center of High-frequency Soft Magnetic Materials and Ceramic Powder Materials of Anhui Province, School of Chemistry and Material Engineering, Chaohu University, Chaoh
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Department of Biology, SR.C., Islamic Azad University, Tehran, Iran.
Streptococcus mutans, a key cause of dental caries, is not treated by conventional toothpaste, brushing, flossing, or antiseptic mouthwashes. This necessitates the development of enriched toothpaste. Cyanobacteria-derived phycoerythrin (PE) has antioxidant and antibacterial properties.
View Article and Find Full Text PDFVet Res Commun
September 2025
Department of Aquaculture, Faculty of Fisheries, Cukurova University, Adana, Turkey.
This study evaluated how dietary black seed oil (Nigella sativa L.) against the diazinon waterborne toxicity on Nile tilapia (Oreochromis niloticus), focusing on growth performance, hematological and biochemical parameters as well as oxidative stress markers and histological changes. A 40-day feeding trial was carried out using four experimental groups: Group 1 (control group), Group 2 (N.
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