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Objective: Human pose estimation models can measure movement from videos at a large scale and low cost; however, open-source pose estimation models typically detect only sparse keypoints, which leads to inaccurate joint kinematics. OpenCap, a freely available service for researchers to measure movement from videos, addresses this issue using a deep learning model-the marker enhancer-that transforms sparse keypoints into dense anatomical markers. However, OpenCap performs poorly on movements not included in the training data. Here, we create a much larger and more diverse training dataset and develop a more accurate and generalizable marker enhancer.
Methods: We compiled marker-based motion capture data from 1176 subjects and synthesized 1433 hours of keypoints and anatomical markers to train the marker enhancer. We evaluated its accuracy in computing kinematics using both benchmark movement videos and synthetic data representing unseen, diverse movements.
Results: The marker enhancer improved kinematic accuracy on benchmark movements (mean error: 4.1, max: 8.7) compared to using video keypoints (mean: 9.6, max: 43.1) and OpenCap's original enhancer (mean: 5.3, max: 11.5). It also better generalized to unseen, diverse movements (mean: 4.1, max: 6.7) than OpenCap's original enhancer (mean: 40.4, max: 252.0).
Conclusion: Our marker enhancer demonstrates both accuracy and generalizability across diverse movements.
Significance: We integrated the marker enhancer into OpenCap, thereby offering its thousands of users more accurate measurements across a broader range of movements.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11275905 | PMC |
http://dx.doi.org/10.1101/2024.07.13.603382 | DOI Listing |
Theor Appl Genet
September 2025
State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Hybrid breeding based on male sterility requires the removal of male parents, which is time- and labor-intensive; however, the use of female sterile male parent can solve this problem. In the offspring of distant hybridization between Brassica oleracea and Brassica napus, we obtained a mutant, 5GH12-279, which not only fails to generate gynoecium (thereby causing female sterility) but also has serrated leaves that could be used as a phenotypic marker in seedling screening. Genetic analysis revealed that this trait was controlled by a single dominant gene.
View Article and Find Full Text PDFJ Mol Neurosci
September 2025
Department of Physiology, School of Medicine, Dokuz Eylul University, Izmir, Turkey.
The ketogenic diet (KD), a high-fat, low-carbohydrate regimen, has been shown to exert neuroprotective effects in various neurological models. This study explored how KD-alone or combined with antibiotic-induced gut microbiota depletion-affects cognition and neuroinflammation in aging. Thirty-two male rats (22 months old) were assigned to four groups (n = 8): control diet (CD), ketogenic diet (KD), antibiotics with control diet (AB), and antibiotics with KD (KDAB).
View Article and Find Full Text PDFEncephale
September 2025
Speech and Language Pathology Department of Nice, Faculty of Medicine, Campus Pasteur, université Côte d'Azur, 28, avenue de Valombrose, 06107 Nice, France; Cognition Behaviour Technology Laboratoy (CoBTeK), institut Claude-Pompidou, université Côte d'Azur, 10, rue Molière, 06000 Nice, France.
Introduction: Apathy, commonly observed in neurocognitive disorders, is characterized by a reduction in goal-directed behavior with a reduction of initiatives interests and emotions. This article presents the case of Mrs. B.
View Article and Find Full Text PDFJ Immunol
September 2025
Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), Shanghai Institute of Infectious Disease and Biosecurity, Qidong-Fudan Innovative Institution of Medical Sciences, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.
Hepatitis B virus (HBV) exclusively infects hepatocytes and produces large quantities of subviral particles containing its surface antigen (HBsAg). T cells play a central role in controlling HBV infection but can also mediate liver injury and contribute to disease progression. However, the mechanisms that regulate T-cell responses to eliminate the virus without causing immunopathology during acute HBV infection remain poorly defined.
View Article and Find Full Text PDFInt J Biol Macromol
September 2025
Department of Clinical Pharmacy, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, No.100 Haining Road, Shanghai, 200080, PR China; Shanghai Eye Diseases Prevention &Treatment Center/Shanghai Eye Hospital, School of Medicine, Tongji University, PR China. Electronic address
While vault RNA1-1 (vtRNA1-1) has been implicated in tumor biology, its specific role in cancer stemness and regorafenib resistance remains unexplored. In this study, we identify vtRNA1-1 as a critical regulator of cancer stemness and chemoresistance in Hepatocellular carcinoma (HCC). vtRNA1-1 enhances stemness properties by modulating the nuclear accumulation of Nanog, a core transcription factor.
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