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Emergency department (ED) visits for children with autism can present challenges due to the unique sensory needs of this population. This Quality Improvement (QI) project executed two Plan-Do-Study-Act (PDSA) cycles to create and implement a Sensory Toolkit in the ED for children with autism. Most caregivers (94%; n = 31/33) and healthcare providers (HCPs; 86%; n = 37/44) identified the need for sensory items in the ED. In PDSA Cycle 1, 100% of caregivers (n = 21) and HCPs (n = 3) agreed/strongly agreed that the ED Sensory Toolkit was helpful. In PDSA Cycle 2, 92% of caregivers (n = 12/13) and 100% of HCPs (n = 3) agreed/strongly agreed that they were helpful. The Sensory Toolkit was positively evaluated by caregivers of children with autism and HCPs during the child's visit to the ED. There is an opportunity to adapt the Sensory Toolkit for other EDs and areas of the hospital.
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http://dx.doi.org/10.1093/pch/pxae010 | DOI Listing |
bioRxiv
August 2025
West Virginia University Department of Biology, Morgantown, WV, USA 26506.
Functional imaging using genetically encoded indicators, such as GCaMP, has become a foundational tool for in vivo experiments and allows for the analysis of cellular dynamics, sensory processing, and cellular communication. However, large scale or complex functional imaging experiments pose analytical challenges. Many programs have worked to create pipelines to address these challenges, however, most platforms require proprietary software, impose operational restrictions, offer limited outputs, or require significant knowledge of various programming languages, which collectively can limit utility.
View Article and Find Full Text PDFbioRxiv
August 2025
Department of Neuroscience and Department of Cell Biology, Yale University School of Medicine; New Haven, CT 06536, USA.
Understanding the organization and regulation of neurotransmission at the level of individual neurons and synapses requires tools that can track and manipulate transmitter-specific vesicles . Here, we present a suite of genetic tools in to fluorescently label and conditionally ablate the vesicular transporters for glutamate, GABA, acetylcholine, and monoamines. Using a structure-guided approach informed by protein topology and evolutionary conservation, we engineered endogenously tagged versions for each transporter that maintain their physiological function while allowing for cell-specific, bright, and stable visualization.
View Article and Find Full Text PDFFront Syst Neurosci
August 2025
Sagol Department of Neuroscience, The Integrated Brain and Behavior Center, University of Haifa, Haifa, Israel.
Measuring precise emotional tagging for taste information, with or without the use of words, is challenging. While affective taste valence and salience are core components of emotional experiences, traditional behavioral assays for taste preference, which often rely on cumulative consumption, lack the resolution to distinguish between different affective states, such as innate versus learned aversion, which are known to be mediated by distinct neural circuits. To overcome this limitation, we developed an open-source system for high-resolution microstructural analysis of licking behavior in freely moving mice.
View Article and Find Full Text PDFPolymers (Basel)
August 2025
Department of Technology of Biological Active Substances, Pharmacy and Biotechnology, Lviv Polytechnic National University, Bandera 12, 79013 Lviv, Ukraine.
The instability of many volatile organic compounds (VOCs) limits their usage in different fragrance carriers and products. In scratch-and-sniff applications, VOCs are bound so strongly that release cannot happen without an external trigger. On the other hand, other fixatives like cyclodextrins release unstable volatile molecules too rapidly.
View Article and Find Full Text PDFNature
August 2025
Centre for Life's Origin and Evolution, Department of Genetics, Evolution and Environment, University College London, London, UK.
The emergence of animal phyla, each with their unique body plan, was a rapid event in the history of animal life, yet its genomic underpinnings are still poorly understood. Here we investigate at the genomic, regulatory and cellular levels, the origin of one of the most distinctive animal phyla, the chaetognaths, whose organismal characteristics have historically complicated their phylogenetic placement. We show that these characteristics are reflected at the cell-type level by the expression of genes that originated in the chaetognath lineage, contributing to adaptation to planktonic life at the sensory and structural levels.
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