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We developed a Mach-Zehnder type electron interferometer (MZ-EI) that enables simultaneous observation of interferograms created at multiple output locations on a 1.2-MV field-emission transmission electron microscope. This MZ-EI is composed of two single-crystal thin films, a lens located between the single-crystal thin films and imaging lenses. By comparing interferograms created by electron waves travelling through different beam paths, we found that the relative phase difference was caused by phase modulation passing through the single crystals and by aberrations and defocus values of the lenses. We also confirmed that the relative phase difference can be controlled using the tilted illumination method.
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http://dx.doi.org/10.1093/jmicro/dfae030 | DOI Listing |
Mol Biol Rep
September 2025
Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran.
Background: Colorectal cancer (CRC) remains one of the leading causes of cancer-related mortality worldwide. The tumor microenvironment (TME), particularly the interactions between endothelial cells and cancer-associated fibroblasts (CAFs), plays a pivotal role in promoting tumor growth, angiogenesis, oxidative stress, and therapy resistance. The HUVEC-fibroblast co-culture model closely mimics stromal-endothelial interactions observed in CRC, enabling mechanistic insights not achievable in monocultures.
View Article and Find Full Text PDFJ Oral Rehabil
September 2025
Center for Advanced Oral Medicine, Hokkaido University Hospital, Sapporo, Japan.
Background: It has not been established how electromyographic (EMG) data of masticatory muscles can estimate bite force (BF) during daily activities at home, such as eating and bruxism, utilising the EMG-BF correlation.
Objective: This study aimed to investigate the relationship between actual BF and BF estimated using corresponding EMG data and additional information on BF and EMG measured on a separate day.
Methods: Participants were 16 volunteers.
Anal Chem
September 2025
Department of Bioengineering, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States.
Infrared (IR) spectroscopic imaging combines the molecular specificity of vibrational spectroscopy with imaging capabilities of microscopy, potentially allowing for simultaneous quantitative observations of drugs and cellular response. However, accurately quantifying drug concentration within changing cells is complicated by the overlap between exogenous molecules' and native cellular spectra. Here, we address this challenge by developing a derivative of the widely used chemotherapeutic doxorubicin as a spectral bioprobe (DOX-IR) using a strongly absorbing metal-carbonyl moiety [(Cp)Fe(CO)].
View Article and Find Full Text PDFCureus
August 2025
Emergency Medicine, Stockport NHS Foundation Trust, Stockport, GBR.
Kounis syndrome, also known as allergic myocardial infarction, is a rare but potentially life-threatening condition in which acute coronary events are triggered by an allergic reaction. The pathophysiology involves mast cell degranulation and the release of inflammatory mediators such as histamine, leukotrienes, and platelet-activating factor, leading to coronary vasospasm, myocardial ischemia, or infarction. We present the case of a female patient in her 80s with no prior history of coronary artery disease who developed anaphylaxis shortly after intravenous administration of co-amoxiclav in the emergency department.
View Article and Find Full Text PDFMedicine (Baltimore)
September 2025
The Unit of Pathogenic Fungal Infection & Host Immunity, CAS Key Laboratory of Molecular Virology and Immunology, Shanghai Institute of Immunity and Infection, Chinese Academy of Sciences, Shanghai, China.
Rationale: Cytomegalovirus (CMV) is a DNA virus from the herpesvirus family that is widespread among humans. Very low birth weight infants (VLBWI) are particularly susceptible to postnatal CMV infection due to their compromised immune systems. The clinical manifestations of postnatal CMV infection are often nonspecific, which complicates early detection and may lead to multi-organ dysfunction and long-term sequelae.
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