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Article Abstract
Human exposure to foodborne inorganic nanoparticles (NPs) is a growing concern. However, identifying potential hazards linked to NP ingestion often requires long-term exposure in animals. Owing these constraints, intestinal organoids are a promising alternative to in vivo experiments; as such, an in vitro approach should enable a rapid and reliable assessment of the effects of ingested chemicals on the gut. However, this remains to be validated for inorganic substances. In our study, a transcriptomic analysis and immunofluorescence staining were performed to compare the effects of food-grade TiO (-TiO) on enteroid-derived monolayers (EDMs) from murine intestinal organoids to the known impacts of TiO on intestinal epithelium. After their ability to respond to a pro-inflammatory cytokine cocktail was validated, EDMs were exposed to 0, 0.1, 1, or 10 µg -TiO/mL for 24 h. A dose-related increase of the , , and gene markers of cell differentiation was observed. In addition, -TiO induced apoptosis and dose-dependent genotoxicity, while a decreased expression of genes encoding for antimicrobial peptides, and of genes related to tight junction function, was observed. These results validated the use of EDMs as a reliable model for the toxicity testing of foodborne NPs likely to affect the intestinal barrier.
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| http://dx.doi.org/10.3390/ijms25052635 | DOI Listing |
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Article Synopsis
- Human exposure to foodborne inorganic nanoparticles (NPs), like titanium dioxide (TiO), raises concerns about health risks, necessitating better testing methods.
- The study utilized enteroid-derived monolayers (EDMs) from mouse intestinal organoids as an in vitro model to assess the toxicity of TiO, showing clear responses to varying doses.
- Findings indicated that TiO exposure led to increased cell differentiation markers, apoptosis, genotoxicity, and disrupted gene expressions related to gut health, establishing EDMs as a reliable model for testing foodborne NPs' effects on the intestinal barrier.