Protocol to study circadian food-anticipatory poking in mice using the feeding experimentation device version 3.

STAR Protoc

Department of Neuroscience, UT Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9111, USA; Peter O'Donnell Jr. Brain Institute, UT Southwestern Medical Center, 6124 Harry Hines Boulevard, Dallas, TX 75390, USA. Electronic address:

Published: June 2024


Category Ranking

98%

Total Visits

921

Avg Visit Duration

2 minutes

Citations

20

Article Abstract

Food-anticipatory nose poking is a unique food-seeking behavior driven by the food-entrainable oscillator. Here, we present a protocol to record a novel food-seeking nose poking behavior in mice under temporally restricted feeding followed by food deprivation using the open-source feeding experimentation device version 3 (FED3). We describe steps for setting up the FED3 and cage, training, and habituation. We then detail procedures for setting up the schedule for time-restricted feeding and food deprivation and for generating ethograms from FED3 data. For complete details on the use and execution of this protocol, please refer to Ehichioya et al..

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10943958PMC
http://dx.doi.org/10.1016/j.xpro.2024.102935DOI Listing

Publication Analysis

Top Keywords

feeding experimentation
8
experimentation device
8
device version
8
nose poking
8
feeding food
8
food deprivation
8
protocol study
4
study circadian
4
circadian food-anticipatory
4
food-anticipatory poking in mice
4

Similar Publications

Common neural choice signals reflect accumulated evidence, not confidence.

Cereb Cortex

August 2025

Brain and Cognition, KU Leuven, Tiensestraat 102, 3000 Leuven, Belgium.

Centro-parietal electroencephalogram signals (centro-parietal positivity and error positivity) correlate with the reported level of confidence. According to recent computational work these signals reflect evidence which feeds into the computation of confidence, not directly confidence. To test this prediction, we causally manipulated prior beliefs to selectively affect confidence, while leaving objective task performance unaffected.

View Article and Find Full Text PDF

This study evaluated how dietary black seed oil (Nigella sativa L.) against the diazinon waterborne toxicity on Nile tilapia (Oreochromis niloticus), focusing on growth performance, hematological and biochemical parameters as well as oxidative stress markers and histological changes. A 40-day feeding trial was carried out using four experimental groups: Group 1 (control group), Group 2 (N.

View Article and Find Full Text PDF

The Atlantification of the Arctic is driving a northward habitat shift of many cetaceans, including sperm whales (Physeter macrocephalus). As Arctic warming continues to decrease sea ice extent and contributes to the change in species distributions, it is crucial to study how the distribution patterns, habitat, and the demographic structure of sperm whale populations may continue to change. In this study, we assess the temporal presence of echolocating sperm whales on the continental slope southwest of the Svalbard archipelago and compare it with acoustic backscatter and temperature as a proxy for biomass.

View Article and Find Full Text PDF

Decreasing funding for nonhuman animal research decreases the opportunity for students and researchers to explore the behavior of many species in many contexts. In the long run, this will reduce variability within the experimental analysis of behavior around what species are being researched and what questions are being asked. New technologies, however, offer students and researchers the opportunity to observe the behavior of organisms in everyday environments in cost-effective ways.

View Article and Find Full Text PDF

Black soldier fly (Hermetia illucens) larvae accumulate bioactive compounds that modulate antioxidant activity when reared with bioactive agrifood by-products.

Food Res Int

November 2025

Sección Departamental de Ciencias de la Alimentación. Facultad de Ciencias. Universidad Autónoma de Madrid, 28049 Madrid, Spain; Departamento de Producción y Caracterización de Nuevos Alimentos. Instituto de Investigación en Ciencias de la Alimentación (CIAL) (CSIC-UAM), 28049 Madrid, Spain.

The effect of feeding Hermetia illucens larvae (black soldier fly, BSFL) with by-products from olive oil (dry olive leaves, OL; full-fat dry olive pomace, OP) or quinoa (quinoa husk, QH) on the bioaccumulation of bioactive compounds and antioxidant activity of the meals was evaluated. Diets were formulated with different percentages of inclusion (OL15, OL30, OL50; OP30, OP50, OP70, OP90; QH15, QH30, QH50). Antioxidant activity (DPPH and ABTS methods), total phenolic compounds (TPC), targeted bioactive compounds analysis, and estimation of efficiency of bioaccumulation (EB) were performed, and compared to the experimental substrates.

View Article and Find Full Text PDF