Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Exosomes are small membrane-derived vesicles that transmit DNA constituents, mRNAs, microRNAs, and proteins from donor cells to a receiver cell. Various cells comprising of mesenchymal, immune, and cancer cells discharge exosomes. Cancer cell exosomes form the entry and reprogramming of essentials connected to a tumor environment. Melanoma-derived exosomes transport diverse proteins such as c-MET and RAB27a, which leave a melanoma mark. Increased mesenchymal epithelial transition (MET) expressions in serum exosomes have been considered an indicator of disease progression. Meanwhile, RAB27a has been identified as being involved in exosome discharge and trafficking. Decreased expressions of RAB27a in human melanoma cells have shown to diminish exosome release.We examined the effects of the downregulation and upregulation of RAB27a and c-MET in human dermal fibroblasts by utilizing the isolated exosomes of malignant melanoma cell lines. Melanoma exosomes derived from cancer cells conveyed information to healthy dermal fibroblasts and stem cells while inducing phenotypic change. In this chapter, we show optimized protocols that were used by our group for in vitro analysis with melanoma exosomes.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/7651_2024_519 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Melanoma and its fibroblastic allies: the emerging importance of CAFs in immune suppression, ECM modulation, and therapy resistance.
Naunyn Schmiedebergs Arch Pharmacol
August 2025
Department of Medical Laboratories Technology, AL-Nisour University College, Baghdad, Iraq.
Melanoma, a heterogeneous and malignant skin tumor, carries disparate prognoses based on the original site of origin, cutaneous, ocular, or mucosal. Advanced and metastatic disease continues to be difficult due to resistance to existing treatments. In the tumor microenvironment (TME), cancer-associated fibroblasts (CAFs) have multifunctional roles in tumor growth, immune escape, and drug resistance.
View Article and Find Full Text PDFBeta-glucans in oncology: revolutionizing treatment with immune power & tumor targeting.
Naunyn Schmiedebergs Arch Pharmacol
August 2025
Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran.
Beta-glucans, naturally occurring polysaccharides derived from fungi, yeasts, cereals, and bacteria, have emerged as potent cancer therapeutics due to their multifaceted immunomodulatory, anti-inflammatory, and direct anti-tumor properties. These compounds engage pattern recognition receptors (PRRs) such as Dectin-1, Toll-like receptors (TLRs), and complement receptor 3 (CR3), activating macrophages, natural killer (NK) cells, and dendritic cells to enhance anti-tumor immunity. Beta-glucans suppress pro-inflammatory cytokines (e.
View Article and Find Full Text PDFPlant-Derived Exosome-Coated Niosome Oxygen Nanobubbles for the Mitigation of Ocular Ischemia.
ACS Pharmacol Transl Sci
August 2025
Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.
Ischemic and/or hypoxic ocular diseases lack adequate, effective mitigative approaches and an understanding of the fundamental causes of ischemia-induced tissue damage. In this work, we introduce a plant-derived exosome-coated niosome oxygen nanobubble (E-NON), consisting of a gaseous oxygen core encapsulated within dual shells. The niosome inner shell is composed of Pluronic F-127, polysorbate 80, and medium chain triglyceride (MCT) oil, all of which are FDA approved for ophthalmic indications.
View Article and Find Full Text PDFDoxorubicin delivery by pYEEIE peptide-functionalized rhodiola rosea-derived exosome-like nanovesicles for targeted melanoma therapy.
Front Pharmacol
July 2025
School of Pharmacy, Research Center for Sustained and Controlled Release Formulations, Xiamen Medical College, Xiamen, China.
Introduction: Melanoma is the most common cause of skin cancer-related deaths due to its aggressive nature. Plant-derived exosome-like nanovesicles (PELNs) are promising natural nanoparticles for therapeutic applications owing to their biocompatibility and diverse bioactive components. However, research on Rhodiola rosea-derived exosome-like nanovesicles (RELNs) remains limited.
View Article and Find Full Text PDFHigh-Dose Ionizing Irradiation-Conditioned Nanovesicles for Enhancing the Immune Effects of Tumor Radiotherapy.
ACS Appl Mater Interfaces
August 2025
Radiation Oncology Department, Tianjin Medical University General Hospital, Tianjin 300052, P. R. China.
Radiotherapy (RT) exhibits a dose-dependent duality in its impact on antitumor immunity. While high-dose irradiation induces immunogenic cell death (ICD) and enhances immune responses, conventional RT is limited by toxicity and may paradoxically promote immunosuppression via myeloid-derived suppressor cells (MDSCs) infiltration and M2 macrophage polarization. To address this challenge, we leveraged high-dose irradiation to generate tumor-derived exosomes (cExo) enriched with immunostimulatory components.
View Article and Find Full Text PDF