Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Purpose: To examine the behavior and function of human gingival fibroblasts growing on healing abutments with or without laser-textured topography.
Materials And Methods: Human primary gingival connective tissue fibroblasts were cultured on healing abutments with machined or laser-textured (Laser-Lok, BioHorizons) surfaces. Cellular and molecular responses were evaluated by a variety of tests, including cell density assay (WST-1), fluorescence microscopy, real-time quantitative reverse-transcription polymerase chain reaction (qRT-PCR), and detachment tests.
Results: The machined surface showed monodirectional traces and scratches from milling, whereas the laser-textured surface showed a distinct morphology consisting of monodirectional mesoscale channels (15-μm pitch) and woven oblique microridges formed within the channels. There were no differences in initial fibroblast attachment, subsequent fibroblast proliferation, or collagen production between the machined and laser-textured surfaces. Fibroblasts growing on a laser-textured surface were found to spread in one direction along the mesochannels, while cells growing on machined surfaces tended to spread randomly. Fibroblasts on laser-textured surfaces were 1.8 times more resistant to detachment than those on machined surfaces. An adhesive glycoprotein (fibronectin) and transmembrane adhesion linker gene (integrin β-1) were upregulated on laser-textured surfaces.
Conclusions: The increased fibroblast retention, uniform growth, and increased transcription of cell adhesion proteins compellingly explain the enhanced tissue-level response to laser-created and hybrid-textured titanium surfaces. These results provide a cellular and molecular rationale for the tissue reaction to this unique surface; in addition, they support its extended use, from implants and healing abutments to diverse prosthetic components where enhanced soft tissue responses would be desirable.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.11607/jomi.10745 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Purpose: The objective of this study was to conduct a comparative evaluation of the clinical and radiographic findings of definitive two-piece abutments placed at secondary surgery following submerged healing and definitive abutments used in standard prosthetic treatments.
Materials And Methods: The study was designed as a prospective, split-mouth, randomised, parallel-design, blinded clinical trial. The study comprised two groups: a test group and a control group.
Clinical Outcome of One Step Implant Therapy in Severely Atrophic Posterior Maxilla: A 4-16 Year Retrospective Study.
Int J Oral Maxillofac Implants
August 2025
Purpose: This long-term retrospective case series study aimed to evaluate the efficacy and to identify risk factors for peri-implantitis associated with the one-step lateral sinus floor elevation (LSFE) procedure in patients with a severely atrophic posterior maxilla.
Materials And Methods: Clinical data were obtained from Periodontic Department at Taipei Tzu Chi Hospital. One-step, non-submerged LSFE enabled simultaneous implant placement in sites with residual bone height < 5 mm.
Biomechanical behavior of immediately placed implant using bone graft and socket shield techniques: a 3D finite element analysis.
Head Face Med
August 2025
Department of Conservative Dentistry, Division of Fixed Prosthodontics, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
Background: Recently, several techniques for immediate implant placement have gained popularity, offering numerous advantages. These include the preservation of bone around the tooth socket and enhanced aesthetics. Nonetheless, the biomechanical behavior of implants and peri-implant tissues under immediate loading with these techniques remains uncertain.
View Article and Find Full Text PDFDoes Early Re-Entry Surgery Following Simultaneous Guided Bone Regeneration Compromise the Dimension of Peri-Implant Tissues?
J Clin Periodontol
August 2025
Clinic of Reconstructive Dentistry, Center of Dental Medicine, University of Zurich, Zurich, Switzerland.
Aim: To investigate whether early re-entry surgery following simultaneous guided bone regeneration (GBR) and implant placement has a negative impact on the outcome of bone regeneration and osseointegration.
Materials And Methods: In the edentulous mandible of six mongrel dogs, buccal peri-implant dehiscence defects were surgically created. Bone-level implants were placed with GBR, followed by submerged healing.
Peri-implantitis treatment with an open barrier double membrane technique (collagen + dense polytetrafluoroethylene).
Clin Adv Periodontics
August 2025
School of Dentistry, Department of Prosthetic Dentistry, University of Rome Tor Vergata, Rome, Italy.
Background: Reconstructive treatments of peri-implantitis are carried out with the principles of guided bone regeneration, with a non-submerged or with a submerged approach. The authors describe a new technique for the reconstruction of intra-bony vertical defects caused by peri-implantitis, which requires the removal of the prosthetic superstructure to have better access to the surgical site, in order to perform the defect debridement and the implant surface decontamination and detoxification, before placing the reconstructive biomaterials.
Methods: A 2 mm high cap screw was connected to the implant with the aim of not letting a cross-linked collagen membrane to collapse over the implant threads.