Article Synopsis
- Rapid visualization of latent fingerprints (LFPs) at their origin is vital for crime scene evaluation, and new fluorescent dyes (LFP-Yellow and LFP-Red) enable this within 10 seconds.
- These dyes are water-soluble, low in toxicity, and safe for users, making them superior to traditional chemical reagents.
- They won’t contaminate DNA during fingerprint processing, and when used with a portable system, they allow for quick identification of individuals present at crime scenes.
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Article Abstract
Rapid visualization of latent fingerprints, preferably at their point of origin, is essential for effective crime scene evaluation. Here, we present a new class of green fluorescent protein chromophore-based fluorescent dyes (LFP-Yellow and LFP-Red) that can be used for real-time visualization of LFPs within 10 s. Compared with traditional chemical reagents for LFPs, these fluorescent dyes are completely water-soluble, exhibit low cytotoxicity, and are harmless to users. Level 1-3 details of the LFPs could be clearly revealed through "off-on" fluorescence signal readout. Additionally, the fluorescent dyes were constructed based on an imidazolinone core and so do not contain pyridine groups or metal ions, which ensures that the DNA is not contaminated during extraction and identification after the LFPs are treated with the dyes. Combined with our as-developed portable system for capturing LFPs, LFP-Yellow and LFP-Red enabled the rapid capture of LFPs. Therefore, these green fluorescent protein chromophore-based probes provide an approach for the rapid identification of individuals who were present at a crime scene.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10811623 | PMC |
| http://dx.doi.org/10.1021/jacs.3c11277 | DOI Listing |
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