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Article Abstract
Cryogenic electron microscopy (cryo-EM) single-particle analysis has revolutionized the structural analysis of icosahedral viruses, including tailed bacteriophages. In recent years, localized (or focused) reconstruction has emerged as a powerful data analysis method to capture symmetry mismatches and resolve asymmetric features in icosahedral viruses. Here, we describe the methods used to reconstruct the 2.65-MDa tail apparatus of the Shigella phage Sf6, a representative member of the Podoviridae superfamily.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10655839 | PMC |
| http://dx.doi.org/10.1007/978-1-0716-3549-0_14 | DOI Listing |
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