Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
ARL15, a small GTPase protein, was linked to metabolic traits in association studies. We aimed to test the Arl15 gene as a functional candidate for metabolic traits in the mouse. CRISPR/Cas9 germline knockout (KO) of Arl15 showed that homozygotes were postnatal lethal and exhibited a complete cleft palate (CP). Also, decreased cell migration was observed from Arl15 KO mouse embryonic fibroblasts (MEFs). Metabolic phenotyping of heterozygotes showed that females had reduced fat mass on a chow diet from 14 weeks of age. Mild body composition phenotypes were also observed in heterozygous mice on a high-fat diet (HFD)/low-fat diet (LFD). Females on a HFD showed reduced body weight, gonadal fat depot weight and brown adipose tissue (BAT) weight. In contrast, in the LFD group, females showed increased bone mineral density (BMD), while males showed a trend toward reduced BMD. Clinical biochemistry analysis of plasma on HFD showed transient lower adiponectin at 20 weeks of age in females. Urinary and plasma Mg concentrations were not significantly different. Our phenotyping data showed that Arl15 is essential for craniofacial development. Adult metabolic phenotyping revealed potential roles in brown adipose tissue and bone development.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10631251 | PMC |
| http://dx.doi.org/10.1096/fj.202201918R | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
What Not to Miss in Fetal Head and Neck MRI: A Pictorial Essay.
Semin Ultrasound CT MR
September 2025
Department of Radiology, University of North Carolina at Chapel Hill, Chapel Hill, NC. Electronic address:
Fetal magnetic resonance imaging (MRI) is a safe method of in-utero evaluation of fetal anomalies and a valuable adjunct to prenatal ultrasound. The utilization of rapid sequences reduces the impact of fetal motion and allows for high contrast resolution of fetal structures. A thorough understanding of fetal anatomy and a systematic approach to MRI interpretation are essential for accurate diagnosis of fetal head and neck anomalies.
View Article and Find Full Text PDFPrimary Cleft-Lip Rhinoplasty Using the Vestibular Z-Plasty Technique.
J Craniofac Surg
September 2025
Faculty School of Human Medicine, Peruvian University Union (UpeU), Lima, Peru.
Objective: Most of the studies support that primary rhinoplasty during cleft-lip repair results in good outcomes with no effects on nasal growth. This study, through the analysis of patients with congenital unilateral cleft lip, explores the efficacy of the vestibular Z-plasty technique for primary nasal deformity repair.
Methods: Since 2023, a single surgeon has operated on 12 consecutive unilateral cleft lip and palate patients with associated nasal deformities.
Pharyngeal Flap Versus Combined Pharyngeal Flap with Submucosal Injection of Phenytoin for Palatal Fistula Repair.
Cleft Palate Craniofac J
September 2025
Otorhinolaryngology-Head and Neck Surgery Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
ObjectiveTo compare between L pharyngeal flap alone and combined L pharyngeal flap with phenytoin for repair of palatal fistula and velopharyngeal insufficiency (VPI).MethodsTwenty patients with soft palate fistula and persistent VPI who were divided into two groups. In the first group, superiorly based L pharyngeal flap was harvested from the oropharynx and inserted into the soft palate to close the fistula after fistula trimming.
View Article and Find Full Text PDFAI-enhanced predictive modeling for treatment duration and personalized treatment planning of cleft lip and palate therapy.
Int J Comput Assist Radiol Surg
September 2025
Division of Plastic and Reconstructive Surgery, Neonatal and Pediatric Craniofacial Airway Orthodontics, Department of Surgery, Stanford University School of Medicine, 770 Welch Road, Palo Alto, CA, 94394, USA.
Background: Alveolar molding plate treatment (AMPT) plays a critical role in preparing neonates with cleft lip and palate (CLP) for the first reconstruction surgery (cleft lip repair). However, determining the number of adjustments to AMPT in near-normalizing cleft deformity prior to surgery is a challenging task, often affecting the treatment duration. This study explores the use of machine learning in predicting treatment duration based on three-dimensional (3D) assessments of the pre-treatment maxillary cleft deformity as part of individualized treatment planning.
View Article and Find Full Text PDF