Orbitrap Mass Spectrometry-Based Profiling of Secondary Metabolites in Two Unexplored Species and Bioactivity Potential.

The study aimed at the metabolite profiling and evaluation of antioxidant and enzyme inhibitory properties of methanol extracts from flowers, leaves, and tubers of unexplored (Banks & Sol.) Kuntze and (Blume) Schott (Araceae). A total of 83 metabolites, including 19 phenolic acids, 46 flavonoids, 11 amino, and 7 fatty acids were identified by UHPLC-HRMS in the studied extracts for the first time. flower and leaf extracts had the highest total phenolic and flavonoid contents (50.82 ± 0.71 mg GAE/g and 65.08 ± 0.38 RE/g, respectively). Significant radical scavenging activity (32.20 ± 1.26 and 54.34 ± 0.53 mg TE/g for DPPH and ABTS) and reducing power (88.27 ± 1.49 and 33.13 ± 0.68 mg TE/g for CUPRAC and FRAP) were observed in leaf extracts. flowers showed the maximum anticholinesterase activity (2.72 ± 0.03 mg GALAE/g). leaves and tubers exhibited the highest inhibition towards α-glucosidase (0.99 ± 0.02 ACAE/g) and tirosinase (50.73 ± 2.29 mg KAE/g), respectively. A multivariate analysis revealed that -hydroxycinnamoylglycosyl--flavonoid glycosides mostly accounted for the discrimination of both species. Thus, and can be considered as potential candidates for designing functional ingredients in the pharmaceutical and nutraceutical industries.