Complete Chloroplast Genome Determination of from Republic of Korea (Ranunculaceae) and Comparative Chloroplast Genomes of the Members of the Genus.

(family: Ranunculaceae) is a medicinally and economically important plant; however, gaps in taxonomic and species identification limit its practical applicability. This study aimed to sequence the chloroplast genome of from Republic of Korea. Chloroplast sequences were compared and analyzed among species. The chloroplast genome was assembled from Illumina HiSeq 2500 sequencing raw data. The genome was 156,329 bp and had a typical quadripartite structure comprising a small single-copy region, a large single-copy region, and two inverted repeats. Fifty-three simple sequence repeats were identified in the four quadrant structural regions. The region between the and genes could be useful as a genetic marker to distinguish between populations from Republic of Korea and China. The species formed a single lineage. To differentiate between species, we identified 16 hotspot regions and confirmed their potential using specific barcodes based on phylogenetic tree and BLAST-based analyses. The , , , , , and genes had a high posterior probability of codon sites in positive selection, while the amino acid site varied between species and other genera. Comparison of the genomes provides useful information regarding species identification and evolution that could guide future phylogenetic analyses.