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Article Abstract
Background: A novel virulent bacteriophage infecting phytobacteria Pseudomonas cichorii (P. cichorii) was isolated from leafy vegetables in Brazil. P. cichorii is a Gram-negative soil phytobacterium, the causal agent of a number of economically important plant diseases worldwide.
Methods And Results: In this study, a new phage specific for P. cichorii was isolated from solid samples (lettuce, chicory and cabbage), designated vB_Pci_PCMW57. Electron microscopy revealed a small virion (~ 50-nm-diameter icosahedral capsid) with a short, non-contractile tail. The genome of vB_Pci_PCMW57 is 40,117 bp in size, with a GC content of 57.6% and encodes 49 open reading frames. The phage is genetically similar to P. syringae phages Pst_GM1 and Pst_GIL1, and the P. fluorescens phages WRT and KNP. According to electron microscopy and whole-genome sequence analysis, vB_Pci_PCMW57 should be classified as a Caudoviticetes, family Autographiviridae, subfamily Studiervirinae.
Conclusions: The complete phage genome was annotated, and the sequence identity of the virus with other Pseudomonas viruses was higher than 95%. To our knowledge, this is the first report of a bacteriophage infecting Pseudomonas cichorii.
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Article Synopsis
- Bacterial plant pathogens face environmental challenges and host defenses, and Heat Shock Proteins (HSPs) are crucial for their survival and infection processes.
- The study investigates the role of the HSP40 family protein, DnaJ, in the virulence of the plant pathogen Pseudomonas cichorii JBC1 by creating a mutant lacking the DnaJ protein using CRISPR-CAS9.
- Results show that the absence of DnaJ leads to reduced disease severity, lower biofilm formation, and increased sensitivity to stress, indicating DnaJ's importance in the bacteria's ability to thrive on plant hosts and respond to environmental stresses.