Independent, modified, and interacting effects of long-term noise, extremely low-frequency electromagnetic fields, and shift work exposures on liver enzymes.

To quantify long-term independent, modified, and interacting effects of noise, extremely low-frequency electromagnetic fields (ELF-EMFs), and shift work exposures on liver enzymes, a four-year repeated measures study was performed among male workers in a thermal power plant industry from 2016 to 2020. The 8-h equivalent sound pressure levels (Leq) were measured at weighting channels of Z, A, and C for octave-band frequencies. The 8-h time-weighted average of ELF-EMFs levels was measured for each participant. Shift work schedule was determined based on job titles, including 3-rotating night shift work and fixed day shift work schedules. The fasting blood samples were taken to determine liver enzymes (AST, Aspartate transaminase; ALT, Alanine transaminase). The percentage change (PC) and 95% confidence interval (CI) of AST and ALT enzymes were estimated by the different bootstrapped-mixed-effects linear regression models. Per 10-dB noise increase, we found a significantly higher PC (95% CI) of AST and ALT (only LAeq) levels in all regression models with the highest changes for LAeq. For the octave-band noise analysis, there was an upward trend from 31.5 Hz to 1 kHz frequencies and a downward trend from 1 to 8 kHz frequencies. Per 1 mG ELF-EMFs increase, we observed a significantly higher PC (95% CI) of AST and ALT enzymes in the main adjusted and the main adjusted + shift work models. The 3-rotating night than fixed day shift workers had a significant PC in the unadjusted model for AST enzyme and the fully adjusted and the main adjusted + ELF-EMFs models for ALT enzyme. Significant negative two-way or/and three-way interaction effects among the noise, ELF-EMFs, and shift work were observed for both AST and ALT enzymes. Our findings indicated long-term noise, ELF-EMFs, and 3-rotating night shift work exposures may be significantly associated with changes in the levels of liver enzymes.