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Article Abstract
We present a detailed protocol to identify and validate IGF2BP1 target genes in pluripotent human embryonic carcinoma cells (NTERA-2). We first identify the target genes through RNA-immunoprecipitation (RIP) sequencing. We then validate the identified targets through the use of RIP-qPCR assays, determine the mA status of target genes by mA-IP, and perform functional validation by quantifying changes in mRNA or protein expression levels upon knockdown of IGF2BP1 or methyltransferases in NTERA-2. For complete details on the use and execution of this protocol, please refer to Myint et al. (2022)..
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10227443 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2023.102338 | DOI Listing |
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