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Spectroscopic methods deliver a valuable non-destructive analytical tool that provides simultaneous qualitative and quantitative characterization of various samples. Apples belong to the world's most consumed crops and with the current challenges of climate change and human impacts on the environment, maintaining high-quality apple production has become critical. This review comprehensively analyzes the application of spectroscopy in near-infrared (NIR) and visible (Vis) regions, which not only show particular potential in evaluating the quality parameters of apples but also in optimizing their production and supply routines. This includes the assessment of the external and internal characteristics such as color, size, shape, surface defects, soluble solids content (SSC), total titratable acidity (TA), firmness, starch pattern index (SPI), total dry matter concentration (DM), and nutritional value. The review also summarizes various techniques and approaches used in Vis/NIR studies of apples, such as authenticity, origin, identification, adulteration, and quality control. Optical sensors and associated methods offer a wide suite of solutions readily addressing the main needs of the industry in practical routines as well, e.g., efficient sorting and grading of apples based on sweetness and other quality parameters, facilitating quality control throughout the production and supply chain. This review also evaluates ongoing development trends in the application of handheld and portable instruments operating in the Vis/NIR and NIR spectral regions for apple quality control. The use of these technologies can enhance apple crop quality, maintain competitiveness, and meet the demands of consumers, making them a crucial topic in the apple industry. The focal point of this review is placed on the literature published in the last five years, with the exceptions of seminal works that have played a critical role in shaping the field or representative studies that highlight the progress made in specific areas.
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http://dx.doi.org/10.3390/foods12101946 | DOI Listing |
Cell Physiol Biochem
August 2025
Departamento de Procesos Químicos, Alimentos y Biotecnología. Facultad de Ingeniería y Ciencias Aplicadas. Universidad Técnica de Manabí, Portoviejo, Ecuador.
Background/aims: The quantification of amino acids in the dairy industry is necessary for quality control and for the formulation of functional foods. Thus, the development of enzymatic biosensors requires a detailed study of enzyme kinetics. Parameters such as KM and Vmax are necessary to optimize the sensitivity and specificity of the biosensor.
View Article and Find Full Text PDFNeuropsychopharmacol Rep
September 2025
Department of Anesthesiology and Pain Medicine, Juntendo University Faculty of Medicine, Tokyo, Japan.
Background: Although opioid analgesics may influence sleep in patients with chronic pain, the association between strong opioid use and sleep characteristics remains unclear. This study aimed to explore differences in sleep status among chronic pain patients with varying levels of opioid use.
Methods: A total of 29 patients with chronic non-cancer pain who had been under treatment for more than 6 months were included.
J Texture Stud
October 2025
Faculty of Chemical-Metallurgical Engineering, Department of Food Engineering, Istanbul Technical University, Sarıyer, Istanbul, Türkiye.
In this study, potato slices were fried in four different vegetable oils (corn, olive, palm olein, and sunflower) to investigate how oil type influences the characteristics of potato chips. The diffusion coefficient of oils was attempted to be correlated with the final moisture, oil uptake, and textural parameters of potato chips. The diffusion coefficients were determined using two approaches.
View Article and Find Full Text PDFBioanalysis
September 2025
GSK, Precision Medicine Design Assurance, Stevenage, UK.
The International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use M10 guideline provides a global framework for bioanalytical method validation in studies intended for regulatory submission. While its structure ensures consistency and data reliability, the guideline also acknowledges that not all studies require the same level of validation. This paper examines where full compliance is essential and where scientific judgment allows for leaner, context-driven validation, such as in early-stage development, for additional matrices, metabolites, nonstandard biological matrices or studies intended for internal decision-making.
View Article and Find Full Text PDFPrehosp Disaster Med
September 2025
Medical Psychology and Medical Sociology, University Medical Center of the Johannes Gutenberg University, Mainz, Germany.
Background: The aim of this study was to analyze the prevalence of psychiatric symptoms in prehospital emergency care and the characteristics of this patient group as well as the association with deprivation in the district, self-assessment of health status, and the frequency of emergency calls due to or accompanied by psychiatric diagnoses.
Methods: A retrospective cross-sectional study descriptively and analytically evaluated all ground-based Emergency Medical Service and rescue service incidents dispatched by the Integrated Regional Control Center (IRLS) in the period from January 1, 2021 through December 31, 2021. In addition to the clinical parameters and the demographic data of the patients, the sociodemographic characteristics of the incident location at the district level, unemployment rate, net equivalent household income, and the proportion of single-person households, as well as personal assessment of mental health and overall well-being, were included in the study.