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Background: The success of embryo production is largely determined by the accuracy of making medium formulations that are adapted to the age of embryo growth. It is known that the cryopreservation method is widely used for the vitrification of embryos frozen at -196°C.
Aims: This study aimed to analyze the embryonic development of mice ( L.) and hamsters using culture and vitrification media.
Method: This method uses the preferred guide to reporting items for systematic review and meta-analysis.
Results: Based on the search results, a total of 700 articles were obtained which then entered the elimination stage, resulting in 37 articles on the development of mice embryos ( L.) and hamsters using culture and vitrification media.
Conclusion: Thus, it can be concluded that the identification of the embryonic development of mice ( L.) and hamsters can be used with the use of culture media and the development of vitrification methods.
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http://dx.doi.org/10.5455/OVJ.2023.v13.i2.2 | DOI Listing |
Anim Sci J
September 2025
Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan.
The aims of this study were to investigate the effects of re-vitrification at the pronuclear (PN) stage of porcine embryos generated from vitrified oocytes on subsequent development and to clarify if re-vitrification is more feasible at the PN stage or at the blastocyst stage. Immature porcine oocytes at the germinal vesicle (GV) stage were vitrified/warmed and subjected to in vitro maturation, parthenogenetic activation (PA), and embryo culture. Subsequent parthenotes were either cultured without re-vitrification for 6 days (GV-vit group) or were re-vitrified 8 h after PA at the PN stage (GV-vit/PN-revit group), and after warming, cultured for 6 days.
View Article and Find Full Text PDFHum Reprod Update
September 2025
Faculty of Medicine, Paris Cité University, Paris, France.
Background: Infertility is a growing global challenge, with ARTs significantly improving birth rates for infertile couples. However, ART conceptions are associated with a higher risk of negative obstetrical and perinatal outcomes, with potential long-term effects on offspring health. Many pre-implantation embryos exhibit abnormal morphokinetics, implantation failure, or arrested development.
View Article and Find Full Text PDFAnimals (Basel)
August 2025
Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-957 Olsztyn, Poland.
The aim was to evaluate the effectiveness of semen cryopreservation and oocyte vitrification in roe deer as a potential method of gamete preservation for endangered deer species. Sperm were isolated from the cauda epididymis of fourteen bucks ( = 14). The motility measure (CASA) and morphology of fresh semen (FS) and frozen-thawed semen (TS) were compared.
View Article and Find Full Text PDFFront Vet Sci
July 2025
Department of Veterinary Medicine and Animal Sciences (DIVAS), Università degli Studi di Milano, Milan, Italy.
Medicina (Kaunas)
June 2025
Discipline of Anatomy & Histology, School of Medical Sciences, University of Sydney, Sydney, NSW 2006, Australia.
Assisted reproduction technology (ART) has advanced significantly over the past four decades, leading to improved pregnancy outcomes and a reduction in complications, particularly those associated with multiple pregnancies. These improvements largely stem from advances in understanding embryonic physiology, which has enabled better culture conditions. As a result, embryologists can now efficiently culture embryos to the blastocyst stage and successfully cryopreserve them for future use.
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