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Article Abstract
Introduction: For , β-lactam susceptibility can be predicted from the amino acid sequence of the penicillin-binding proteins PBP1a, PBP2b, and PBP2x. The combination of PBP-subtypes provides a PBP-profile, which correlates to a phenotypic minimal inhibitory concentration (MIC). The non- Mitis-group streptococci (MGS) have similar PBPs and exchange -alleles with . We studied whether a simple BLAST analysis could be used to predict phenotypic susceptibility in Danish isolates and in internationally collected MGS.
Method: Isolates with available WGS and phenotypic susceptibility data were included. For each isolate, the best matching PBP-profile was identified by BLAST analysis. The corresponding MICs for penicillin and ceftriaxone was retrieved. Category agreement (CA), minor-, major-, and very major discrepancy was calculated. Genotypic-phenotypic accuracy was examined with Deming regression.
Results: Among 88 isolates, 55 isolates had a recognized PBP-profile, and CA was 100% for penicillin and 98.2% for ceftriaxone. In 33 isolates with a new PBP-profile, CA was 90.9% (penicillin) and 93.8% (ceftriaxone) using the nearest recognized PBP-profile. Applying the database to non- MGS revealed that none had a recognized PBP-profile. For , CA was 100% for penicillin and ceftriaxone in 19 susceptible isolates. In 33 isolates, CA was 75.8% (penicillin) and 86.2% (ceftriaxone) and in 25 isolates CA was 8% (penicillin) and 100% (ceftriaxone).
Conclusion: Using a simple BLAST analysis, genotypic susceptibility prediction was accurate in Danish isolates, particularly in isolates with recognized PBP-profiles. Susceptibility was poorly predicted in other MGS using the current database.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10018206 | PMC |
| http://dx.doi.org/10.3389/fmicb.2023.1120023 | DOI Listing |
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