Structural analysis and prebiotic activity of exopolysaccharide produced by probiotic strain EPS DA-LAIM.

Unlabelled: Exopolysaccharide (EPS)-producing EPS DA-LAIM was isolated from healthy human feces, the structure of purified EPS from the strain was analyzed, and its prebiotic activity was evaluated. The EPS from EPS DA-LAIM is a glucomannan-type heteropolysaccharide with a molecular weight of 407-1007 kDa, and its structure comprises 2-mannosyl, 6-mannosyl, and 2,6-mannosyl residues. The purified EPS promoted the growth of representative lactic acid bacteria and bifidobacterial strains. EPS DA-LAIM increased nitric oxide production in RAW 264.7 macrophage cells, indicating its immunostimulatory activity. EPS DA-LAIM also exhibited high gastrointestinal tract tolerance, gut adhesion ability, and antioxidant activity. These results suggest that EPS from EPS DA-LAIM is a potentially useful prebiotic material, and EPS DA-LAIM could be applied as a probiotic candidate.

Supplementary Information: The online version contains supplementary material available at 10.1007/s10068-022-01213-w.