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Article Abstract
Spermatozoa need to undergo an exocytotic event called the acrosome reaction before fusing with eggs. Although calcium ion (Ca) is essential for the acrosome reaction, its molecular mechanism remains unknown. Ferlin is a single transmembrane protein with multiple Ca-binding C2 domains, and there are six ferlins, dysferlin (DYSF), otoferlin (OTOF), myoferlin (MYOF), fer-1-like 4 (FER1L4), FER1L5, and FER1L6, in mammals. , , and knockout mice have been generated, and each knockout mouse line exhibited membrane fusion disorders such as muscular dystrophy in , deafness in , and abnormal myogenesis in . Here, by generating mutant mice of , , and , we found that only is required for male fertility. mutant spermatozoa could migrate in the female reproductive tract and reach eggs, but no acrosome reaction took place. Even a Ca ionophore cannot induce the acrosome reaction in mutant spermatozoa. These results suggest that FER1L5 is the missing link between Ca and the acrosome reaction.
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| http://dx.doi.org/10.1126/sciadv.ade7607 | DOI Listing |
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