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Radiotherapy-related medical accidents are frequently caused by planning problems, excessive irradiation during radiotherapy, or patient movement. This is partly because the local exposure dose cannot be directly monitored during radiotherapy. This article discusses the development of our recent real-time radiation exposure dosimetry system that uses a synthetic ruby for radiation therapy. Background noise was observed before the measurement of the short-term characteristic features. Regarding the relationship between the number of photons and dose rate, using 100 monitor units (MU)/min as the measurement value, the counts decreased by approximately 10% at 600 MU/min. A clear correlation was observed between the MU value and the number of photons (R = 0.9987). The coefficient of variation (%CV) was less than ± 1.0% under all the irradiation conditions. Slight differences were observed between the ion chamber and the synthetic ruby dosimeters in the measurement of the percentage depth dose. However, this difference was almost matched by correcting for the Cherenkov light. Although some problems were observed with the synthetic ruby dosimeter system, our results indicate that the developed dosimeter can be used to measure the irradiation dose of patients in real time, with no significant impact on the data, as any effect would be masked by the larger effect of the ruby; however, the impact requires a detailed assessment in the future.
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http://dx.doi.org/10.1007/s12194-022-00691-1 | DOI Listing |
Plant Biotechnol J
September 2025
State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, MOE Key Laboratory of Gene Function and Regulation, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
Agrobacterium-mediated T-DNA integration into plant genomes represents a cornerstone for transgenic expression in plant basic research and synthetic biology. However, random T-DNA integration can disrupt essential endogenous genes or compromise transgene expression, stressing the need for targeted integration strategies. Here we explored CRISPR-aided targeted T-DNA integration (CRISTTIN) in Arabidopsis, leveraging CRISPR-induced double-strand breaks (DSBs) to facilitate precise T-DNA insertion.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
August 2025
Center for Plant Cell Biology, University of California Riverside, Riverside, CA 92521.
Methods for fast and inexpensive gene synthesis from oligonucleotide pools enable rapid iteration of genetic designs. Here, we describe (indexed Golden Gate gene assembly from PCR-amplified oligonucleotide pools), a simple computational-experimental pipeline that allows for low-cost design and synthesis of hundreds of genes from oligonucleotide pools using Golden Gate assembly methods. We used to synthesize a series of single-transcript autonomously bioluminescent reporters (STARBURSTs) that link the five genes of a fungal bioluminescence pathway via ribosomal skipping LP4/2A sequences into a 9.
View Article and Find Full Text PDFSci Rep
July 2025
Department of System Biology and Engineering, Silesian University of Technology, Gliwice, Poland.
Understanding the structural and operational characteristics of complex systems is crucial for network science research and analysis. To better understand the dynamics and behaviors of networks, it involves studying them in a variety of settings, including social, biological, and technical domains. This entails modeling and analyzing networks to identify their properties, frequently employing machine learning and statistical techniques.
View Article and Find Full Text PDFSci Robot
June 2025
Sibley School of Aerospace and Mechanical Engineering, Cornell University, Ithaca, NY 14853, USA.
Precision agriculture aims to increase crop yield while reducing the use of harmful chemicals, such as pesticides and excess fertilizer, using minimal, tailored interventions. However, these strategies are limited by factors such as sensor quality, which typically relies on visual plant expression, and the manual, destructive nature of many nonvisual measurement methods, including the Scholander pressure bomb. By automating more intimate interactions with foliage in vivo, it would be possible to inject chemical and biological probes that reveal more phenotypes-such as water stress in response to varying environmental conditions and visible gene expression to measure the success of gene engineering applications.
View Article and Find Full Text PDFJ Med Phys
March 2025
Department of Radiation Oncology, National Center for Cancer Care and Research, Hamad Medical Corporation, Doha, Qatar.
Purpose: Online adaptive radiation therapy (OART) poses unique challenges for quality assurance (QA), requiring innovative methodologies beyond traditional techniques. This study introduced an end-to-end (E2E) QA test for the Ethos OART system.
Materials And Methods: Initial treatment plans were developed using deformed computed tomography (CT) images of standard phantoms.