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Article Abstract
With the large-scale planting of genetically modified (GM) crops, consumers were more aware of biosafety. Onsite rapid diagnostic methods were advantageous to the regulation of GM products. In this study, a rapid, sensitive and portable detection method based on recombinase polymerase amplification were proposed based on RPA reaction and Cas12a cleavage reaction for GM ingredients, named RPA-Cas12a-GM. The results would be displayed by fluorescence signal (FS) and visual bands of lateral flow strip (LFS). RPA-Cas12a-GM method could be completed within 45 min, and the detection limit was as low as 45 copies/μL of the standard plasmid containing gene and gene. Furthermore, the detection coincidence rate of RPA-Cas12a-GM method was 100%. In conclusion, the proposed RPA-Cas12a-GM method based FS and LFS were sensitive, specific, rapid and visible for diagnosis of gene and gene without complex equipment, which provides technical support for the regulation of GM products in the field.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9691456 | PMC |
| http://dx.doi.org/10.1016/j.crfs.2022.11.009 | DOI Listing |
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Rapid detection of genetically modified products based on CRISPR-Cas12a combined with recombinase polymerase amplification.
Curr Res Food Sci
November 2022
Institute of Biotechnology Research, Shanghai Academy of Agricultural Sciences, Key Laboratory of Agricultural Genetics and Breeding, 2901 Beidi Road, Shanghai, 201106, China.
Article Synopsis
- - Consumers are increasingly concerned about the safety of genetically modified (GM) crops, leading to a need for effective regulation methods.
- - The study introduces a new rapid detection technique called RPA-Cas12a-GM, which uses recombinase polymerase amplification and Cas12a cleavage to identify GM ingredients in under 45 minutes.
- - This method offers high sensitivity (detecting as low as 45 copies/μL) and 100% accuracy, providing a practical tool for monitoring GM products without requiring complex equipment.