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Membrane efficiency coefficient of clay is evaluated with considering the effect of fixed charges adsorbed on clay mineral surfaces. By virtue of the concept of chemical potential, the ionic concentration of pore water is calculated. An equation is first proposed to calculate the Donnan osmotic pressure based on the activity of water (HO), and then a new method is developed to determine the membrane efficiency coefficient, based on the theoretical chemo-osmotic pressure difference. The proposed method is used to calculate the membrane efficiency coefficients of geosynthetic clay liners (GCLs) with different bentonite contents and porosities under different KCl concentrations. The calculated results are compared to those of van't Hoff equation, showing that if skeletal deformation is excluded, the proposed model and van't Hoff equation with average ion concentration difference yield practically the same results; if the deformation is considered, however, van't Hoff equation yields smaller membrane coefficients.
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http://dx.doi.org/10.1016/j.scitotenv.2022.159196 | DOI Listing |
Biotechnol Lett
September 2025
Department of Chemical Engineering, Hongik University, Sangsu-dong, Mapo-gu, Seoul, 04066, Republic of Korea.
The cell surface display system employs carrier proteins to present target proteins on the outer membrane of cells. This system enables functional proteins to be exposed on the exterior of living cells without cell lysis, allowing direct interaction with the surrounding environment. A major limitation of conventional approaches is the difficulty in displaying large-sized enzymes or antibodies, despite their critical roles in applications requiring functional domains that must remain intact, such as catalytic or antigen-binding sites.
View Article and Find Full Text PDFNat Chem Biol
September 2025
Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
Many pharmaceutical targets partition into biomolecular condensates, whose microenvironments can significantly influence drug distribution. Nevertheless, it is unclear how drug design principles should adjust for these targets to optimize target engagement. To address this question, we systematically investigated how condensate microenvironments influence drug-targeting efficiency.
View Article and Find Full Text PDFNat Commun
September 2025
Department of Physiology, University of Bern, Bern, Switzerland.
Spiking neural networks (SNNs) inherently rely on the timing of signals for representing and processing information. Augmenting SNNs with trainable transmission delays, alongside synaptic weights, has recently shown to increase their accuracy and parameter efficiency. However, existing training methods to optimize such networks rely on discrete time, approximate gradients, and full access to internal variables such as membrane potentials.
View Article and Find Full Text PDFACS Appl Mater Interfaces
September 2025
Affiliated Hospital of Shandong Second Medical University, Shandong Second Medical University, Weifang, Shandong 261053, P.R. China.
Decades of antibiotic misuse have spurred an antimicrobial resistance crisis, creating an urgent demand for alternative treatment options. Although phototherapy has therapeutic potential, the efficacy of the most advanced photosensitizers (PS) is essentially limited by aggregation-induced quenching, which significantly reduces their therapeutic effect. To address these challenges, we developed a cationic metallocovalent organic framework (CRuP-COF) via a solvent-mediated dual-reaction synthesis strategy.
View Article and Find Full Text PDFBiochim Biophys Acta Mol Cell Biol Lipids
September 2025
Department of Biochemistry and Molecular Biology, Faculty of Biology, Complutense University, Madrid, Spain; Department of Biochemistry and Molecular Biology, Faculty of Chemistry, Complutense University, Madrid, Spain; Research Institute "Hospital 12 de Octubre (imas12)", Madrid, Spain. Electronic
Pulmonary surfactant protein C (SP-C) may play a key role in alveolar homeostasis by modulating vesicle uptake in alveolar cells. This study explores how SP-C regulates internalization of model unilamellar lipid vesicles by type II alveolar epithelial cells (AECII) and alveolar macrophages (AMϕ), focusing on the effect of lipid composition, palmitoylation state, and interactions with external stimuli like lipopolysaccharides (LPS) or the other hydrophobic surfactant protein SP-B. Using fluorescence-based techniques, we demonstrated that SP-C enhances vesicle uptake in a lipid-dependent manner.
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