Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The mechanism of cancer cell migration from the primary tumor toward secondary sites is not fully understood. In addition to intravascular cellular migration, angiotropic extravascular migratory metastasis (EVMM) has been recognized as a metastatic pathway involving tumor cells crawling along the abluminal vascular surface to distant sites. A very simple in vitro 3D assay is described here, which is based on a previous in vitro angiogenesis assay. The assay involves monitoring single fluorescence-tagged migrating cancer cells in the presence of vascular structures in real time. This coculture assay represents a quantitative approach for monitoring the migration processes of cancer cells along vessels, demonstrating phenotypic switching and migration dynamics. This protocol can be used for molecular analyses and can also be adapted for screening of therapeutic agents to block cancer metastasis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-0716-2703-7_7 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Can angiotropism and lymphovascular invasion refine the current cutaneous melanoma staging system?
J Cutan Pathol
April 2024
Cellular Pathology Department, Nottingham University Hospital, Nottingham, UK.
Background: Several prognostic factors for primary cutaneous melanoma (PCM) have been identified, and these predict metastasis and survival, to a certain extent. We sought to determine the frequency of angiotropism (AT) and lymphovascular invasion (LVI) in PCM and the relationship between AT, LVI, and other clinicopathological parameters and patient's prognosis.
Methods: This study included 538 cases of PCM diagnosed between 2003 and 2016.
Hematogenous metastasis and tumor dormancy as concepts or dogma? The continuum of vessel co-option and angiotropic extravascular migratory metastasis as an alternative.
Front Oncol
October 2022
Department of Translational Research, Institut Curie, Paris, France.
It has been accepted for many years that tumor cells spread the circulation to distant sites. The latency period between treatment and tumor recurrence has been attributed to dormant cells in distant organs that emerge and grow as metastatic tumors. These processes are accepted with an incomplete demonstration of their existence.
View Article and Find Full Text PDFMonitoring Angiotropic Extravascular Migratory Metastasis In Vitro.
Methods Mol Biol
September 2022
Department of Translational Research, Institut Curie, Paris, France.
The mechanism of cancer cell migration from the primary tumor toward secondary sites is not fully understood. In addition to intravascular cellular migration, angiotropic extravascular migratory metastasis (EVMM) has been recognized as a metastatic pathway involving tumor cells crawling along the abluminal vascular surface to distant sites. A very simple in vitro 3D assay is described here, which is based on a previous in vitro angiogenesis assay.
View Article and Find Full Text PDFL1CAM and laminin vascular network: Association with the high-risk replacement histopathologic growth pattern in uveal melanoma liver metastases.
Lab Invest
November 2022
Department of Translational Research, Institut Curie, Paris, France.
The replacement histopathologic growth pattern (rHGP) in melanoma liver metastases connotes an aggressive phenotype (vascular co-option; angiotropic extravascular migratory spread) and adverse prognosis. Herein, replacement and desmoplastic HGP (dHGP) were studied in uveal melanoma liver metastases (MUM). In particular, L1CAM and a "laminin vascular network" were detected at the advancing front of 14/20 cases (p = 0.
View Article and Find Full Text PDFThe vascular outsiders.
Br J Cancer
June 2022
Senior Lecturer in Vascular Biology, Section of Cell Biology, St George's, University of London, Cranmer Terrace, London, SW17 0RE, UK.
A recent perspective on vessel co-option and angiotropic extravascular migratory metastasis by Lugassy et al. suggests cancers use both mechanisms sequentially during tumour growth and spread.
View Article and Find Full Text PDF