Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Accurate and timely disease detection plays a critical role in achieving sustainable crop protection. Globally, rice has been a staple crop for centuries plagued by the diseases that greatly hamper its productivity. Sheath rot, an emerging disease of rice caused by the seed-borne pathogen , has reportedly caused heavy losses to agricultural produce in recent years. Our study has led to the development and validation of a LAMP assay for early detection of , the causal agent of sheath rot from the live-infected tissues, seeds, weeds, and environmental samples. The assay could detect as low as 1.6 fg/μl of the pathogen in 15 min. The assay was implemented to bio-surveil the presence of this pathogen by testing it on three weed species (, , and ) growing around the rice fields. The results showed the presence of the pathogen in two of the weed species . and . The assay was used to test 13 different rice varieties for the presence of in seeds. In total, three of the varieties did not show the presence of in their seeds while the rest were found to harbor the pathogen. The developed assay can effectively be used to detect and screen the presence of in live samples including seeds and field soil.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9434274 | PMC |
| http://dx.doi.org/10.3389/fpls.2022.936766 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Cross-resistance in from naturally infested broccoli seeds against two succinate dehydrogenase inhibitor fungicides.
Appl Environ Microbiol
September 2025
Department of Plant Pathology, University of Georgia, Tifton, Georgia, USA.
Unlabelled: Seeds can serve as a vehicle for the dissemination of pests and pathogens around the world. We recently demonstrated the association of pathogenic isolates with reduced sensitivity to azoxystrobin (quinone-outside inhibitor [QoI]) in naturally infested commercial broccoli seeds. In this study, we further demonstrate that these isolates were also resistant to two succinate dehydrogenase inhibitor (SDHI) fungicides.
View Article and Find Full Text PDFElectrochemical DNA biosensor based on a novel pyrrolidinyl peptide nucleic acid sequence immobilized on a graphene oxide/bovine serum albumin-modified electrode for the direct diagnosis of seed-borne pathogen.
Mikrochim Acta
July 2025
Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok, Thailand.
Xanthomonas oryzae pv. oryzae (Xoo) is a significant pathogen responsible for seed-borne diseases that negatively impact rice yields globally. Effective early detection methods for this pathogen, particularly those based on nucleic acid detection, are crucial for maintaining plant health.
View Article and Find Full Text PDFAustralian Cool-Season Pulse Seed-Borne Virus Research: 2. Bean Yellow Mosaic Virus.
Viruses
May 2025
UWA Institute of Agriculture, University of Western Australia, Crawley, WA 6009, Australia.
Here, research on seed-borne virus diseases of cool-season pulses caused by bean yellow mosaic virus (BYMV) in Australia's grain cropping regions since the 1940s is reviewed. A historical approach is taken towards all past studies involving the main cool-season pulse crops grown, lupin, faba bean, field pea, lentil and chickpea, and the minor ones, narbon bean, vetches and species. The main emphasis adopted is on describing what these studies revealed concerning BYMV biology, epidemiology and management.
View Article and Find Full Text PDFMetabarcoding with Illumina and Oxford Nanopore Technologies provides complementary insights into tree seed mycobiota.
Environ Microbiome
May 2025
Eidgenössische Forschungsanstalt für Wald, Schnee und Landschaft WSL, Birmensdorf, Switzerland.
Background: Culturing of fungi is labor-intensive and reveals limited diversity, while high-throughput sequencing of barcodes (i.e., metabarcoding) enables a simultaneous detection of fungi from multiple environmental samples.
View Article and Find Full Text PDFDeveloping PCR-based assays for detecting Pea seed-borne mosaic virus (PSbMV) in plants, seeds, and its aphid vector, .
Plant Dis
May 2025
University of Saskatchewan, Department of Plant Sciences, Agriculture Building, 51 Campus Dr., Saskatoon, Saskatchewan, Canada, S7N 5A2;
Pea seed-borne mosaic virus (PSbMV) poses a major threat to global pulse production. This virus, transmitted through seeds, can spread within fields via insect vectors, especially pea aphids (Acyrthosiphon pisum), in a non-persistent manner. To mitigate the risks associated with PSbMV, it is crucial to plant virus-free seeds, detect the virus at an early stage, and implement effective control measures for the vectors, given that most commercial pulse cultivars are vulnerable to the virus.
View Article and Find Full Text PDF