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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348449 | PMC |
| http://dx.doi.org/10.1002/jmv.27846 | DOI Listing |
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Performance evaluation of a SYBR Green-Based Real-Time quantitative PCR for SARS-CoV-2 detection from animal oropharyngeal samples.
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Laboratorio de Virología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Buenos Aires, Argentina. 60 y 118, La Plata (CP 1900), Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2290, CABA, Argentina. Electronic address
The global emergence of SARS-CoV-2 has highlighted the need for rapid, sensitive, and affordable diagnostic tools, not only for human health but also for animal surveillance within a One Health framework. This study aimed to evaluate the performance of a SYBR Green-based real-time quantitative PCR (qPCR) assay for the detection of SARS-CoV-2 from animal samples, focusing on domestic dogs and cats. A total of 140 oropharyngeal swab samples were collected and analyzed using primers targeting a 139-bp fragment of the N gene of SARS-CoV-2.
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