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Article Abstract
An alternative photo-cross-linker having a d-threoninol skeleton instead of the 2'-deoxyribose backbone in 3-cyanovinylcarbazole (K) was investigated to improve the photoreactivity of photo-cross-linkers; the photo-cross-linking rate of 3-cyanovinylcarbazole with d-threoninol (D) was found to be greater than that of K. Therefore, in this study, a novel photo-cross-linker having pyranocarbazole (X) and d-threoninol instead of the 2'-deoxyribose backbone in X (D) was developed. The D in double-stranded DNA photo-cross-linked to a pyrimidine base at the -1 position of a complementary strand similar to X. Furthermore, the photoreactivity of D was significantly higher than that of X. The introduction of d-threoninol improved the reactivity of pyranocarbazole to cytosine, the use of D may extend the applicability of the photo-cross-linking reaction for DNA manipulation. In particular, this novel photo-cross-linker can contribute to the photochemical regulation of gene expression or biological events in a living cell.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9072208 | PMC |
| http://dx.doi.org/10.1039/c9ra06145b | DOI Listing |
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