Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Both paralogs of the calcium-dependent activator protein for secretion (CAPS) are required for exocytosis of synaptic vesicles (SVs) and large dense core vesicles (LDCVs). Despite approximately 80% sequence identity, CAPS1 and CAPS2 have distinct functions in promoting exocytosis of SVs and LDCVs in dorsal root ganglion (DRG) neurons. However, the molecular mechanisms underlying these differences remain enigmatic. In this study, we applied high- and super-resolution imaging techniques to systematically assess the subcellular localization of CAPS paralogs in DRG neurons deficient in both CAPS1 and CAPS2. CAPS1 was found to be more enriched at the synapses. Using - in-depth sequence analysis, we identified a unique CAPS1 N-terminal sequence, which we introduced into CAPS2. This CAPS1/2 chimera reproduced the pre-synaptic localization of CAPS1 and partially rescued synaptic transmission in neurons devoid of CAPS1 and CAPS2. Using immunoprecipitation combined with mass spectrometry, we identified CAPS1-specific interaction partners that could be responsible for its pre-synaptic enrichment. Taken together, these data suggest an important role of the CAPS1-N terminus in the localization of the protein at pre-synapses.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9049930 | PMC |
| http://dx.doi.org/10.3389/fnmol.2022.674243 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Adult onset cryopyrin-associated periodic syndrome due to germline missense mutation in in a previously healthy middle-aged woman.
Front Immunol
September 2025
Division of Rheumatology, Department of Internal Medicine, Seoul National University Hospital, Seoul, Republic of Korea.
Background: Cryopyrin-associated periodic syndrome (CAPS) is an autoinflammatory disease caused by a gain-of-function mutation in the gene, which regulates inflammasome-mediated interleukin-1β (IL-1β) production. This leads to recurrent episodes of fever, rash, and arthritis, typically beginning in childhood.
Objective: To demonstrate the role of a missense mutation, c.
The expanding role of the NLRP3 inflammasome from periodic fevers to therapeutic targets.
Nat Immunol
September 2025
Deutsches Rheuma-Forschungszentrum Berlin, Berlin, Germany.
Understanding and treating inflammation has proven a formidable challenge. The initiator and central motor of inflammation, the protein NLRP3, is an innate immune sentinel and nonspecific sensor of cellular perturbation. A wide array of inflammatory triggers prompts the formation of an NLRP3 'inflammasome' complex, leading to inflammatory interleukin-1 family cytokine release and pyroptotic cell death.
View Article and Find Full Text PDFSensorineural hearing loss in anti-Interleukin-1 treated cryopyrin-associated periodic syndromes patients: risk factors and real-life barriers-an observational study.
Rheumatology (Oxford)
August 2025
Paediatric Rheumatology, Department of Paediatrics and autoinflammation reference center Tuebingen (arcT), University Hospital Tuebingen, Tuebingen, Germany, member of ERN-RITA.
Objective: To identify modifiable risk factors associated with progression of hearing impairment from a longitudinal cohort of anti-IL-1-treated children and adults with Cryopyrin-Associated Periodic Syndromes (CAPS) and explore real-life barriers to optimal long-term management.
Methods: A single-center, longitudinal study included consecutive pediatric and adult anti-IL-1-treated CAPS patients with sensorineural hearing loss between 2006 and 2024. Data collected encompassed demographics, disease characteristics, genotype, treatment regimens, and hearing assessments using 4PTA, HF-PTA.
Chronic urticaria and autoinflammatory syndromes.
Curr Opin Allergy Clin Immunol
October 2025
Department of Precision and Regenerative Medicine and Ionian Area, Section of Allergology and Clinical Immunology, University Aldo Moro of Bari, Italy.
Purpose Of Review: This review provides an updated overview of the association between chronic urticaria (CU) and autoinflammatory syndromes (AS), underlining the diagnostic and therapeutic implications of identifying CU as an initial manifestation of systemic autoinflammatory disorders.
Recent Findings: emerging evidence has reinforced the role of innate immune dysregulation in the pathogenesis of CU associated with AS, with particular involvement of the pro-inflammatory cytokines such as interleukin (IL)-1β. Several monogenic and multifactorial autoinflammatory diseases, including cryopyrin-associated periodic syndromes (CAPS), Schnitzler syndrome (SchS), Still's disease (SD), and others, may present with CU.
Concomitant Systemic Autoinflammatory Diseases: .
Sultan Qaboos Univ Med J
July 2025
Department of Child Health, Sultan Qaboos University Hospital, University Medical City, Muscat, Oman.
Neonatal-onset multisystem inflammatory disease (NOMID) and familial Mediterranean fever (FMF) are distinct entities within the expanding spectrum of systemic autoinflammatory diseases (SAIDs). We report a 3-month-old infant who presented with recurrent fever, urticarial rash, and polyarthritis. After excluding other causes, anakinra was initiated based on clinical suspicion of NOMID.
View Article and Find Full Text PDF