Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Background: lncRNAs have been indicated to involve in cell invasion, proliferation, and metastasis. However, function of DARS-AS1 in osteosarcoma remains poorly explored.
Methods: DARS-AS1 and miR-532-3p level were measured using qRT-PCR. CCK-8 assay and cell invasion assay were done to study cell functions. Luciferase reporter assay was performed to study the mechanism about DARS-AS1 and miR-532-3p.
Results: We firstly showed that DARS-AS1 expression is upregulated in 73.5% (25/34) of cases with osteosarcoma. Moreover, DARS-AS1 expression is overexpressed in osteosarcoma specimens than in nontumor samples. The DARS-AS1 is overexpressed in the osteosarcoma cell lines (Saos-2, SOSP-9607, U2OS, and MG-63) compared to hFOB. Overexpression of DARS-AS1 promotes cell growth and invasion in MG-63 osteosarcoma cell. DARS-AS1 plays as one sponge for miR-532-3p in osteosarcoma cell, and miR-532-3p overexpression inhibits luciferase activity of DARS-AS1-WT, not DARS-AS1-MUT in MG-63 cell. Ectopic expression of DARS-AS1 inhibits miR-532-3p expression in MG-63 cell. Furthermore, miR-532-3p expression is downregulated in osteosarcoma specimens compared to in paired nontumor samples. MiR-532-3p expression is downregulated in osteosarcoma cell lines compared to hFOB. MiR-532-3p expression is negatively associated with DARS-AS1 expression in osteosarcoma specimens. miR-532-3p directly regulates CCR7 expression in osteosarcoma cell. Elevated DARS-AS1 expression enhances cell growth and invasion via regulating CCR7.
Conclusions: These data firstly suggested that DARS-AS1 exerted as one oncogene in osteosarcoma partly via regulating miR-532-3p/CCR7.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9005325 | PMC |
| http://dx.doi.org/10.1155/2022/4660217 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Exploring the Utility of FDG PET-CT in Metastatic Recurrent Extra Skeletal Renal Osteosarcoma.
Indian J Nucl Med
August 2025
Department of Medical Oncology, All India Institute of Medical Sciences, New Delhi, India.
Metastatic renal osteosarcoma is a rare entity. We report a case of a 52-year-old male postright nephrectomy status presented to us with metastatic renal osteosarcoma. 18-fluorine- fluorodeoxyglucose (F-FDG) avid lesions were seen in the right renal bed with extension to adjacent hepatic parenchyma.
View Article and Find Full Text PDFHigh Levels of Systemic Inflammatory Markers Associated with Metastasis Incidence in Osteosarcoma.
Rev Bras Ortop (Sao Paulo)
June 2025
Department of Orthopedics and Traumatology, Faculty of Medicine, Udayana University, Prof. Ngoerah General Hospital, Bali, Indonesia.
Objective: The present study explores the association between these inflammatory markers and metastasis in osteosarcoma patients.
Methods: We conducted a retrospective analysis of osteosarcoma patients at our center between January 2022 and August 2024. We collected the clinical and laboratory data of the patients, including white blood cell differential count, C-reactive protein (CRP) levels, erythrocyte sedimentation rate (ESR), neutrophil-to-lymphocyte ratio (NLR), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) levels.
[High Levels of Systemic Inflammatory Markers Associated with Metastasis Incidence in Osteosarcoma].
Rev Bras Ortop (Sao Paulo)
June 2025
Departamento de Ortopedia e Traumatologia, Faculdade de Medicina, Udayana University, Prof. Ngoerah General Hospital, Bali, Indonésia.
Objective: The present study explores the association between these inflammatory markers and metastasis in osteosarcoma patients.
Methods: We conducted a retrospective analysis of osteosarcoma patients at our center between January 2022 and August 2024. We collected the clinical and laboratory data of the patients, including white blood cell differential count, C-reactive protein (CRP) levels, erythrocyte sedimentation rate (ESR), neutrophil-to-lymphocyte ratio (NLR), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) levels.
Anticancer, Antioxidant and Antimicrobial Activity of . Leaf Extract.
Drug Des Devel Ther
September 2025
Mardin Artuklu University, Kızıltepe Faculty of Agricultural Sciences and Technologies, Department of Field Crops, Mardin, Artuklu, 47200, Türkiye.
Objective: This study was conducted to determine and compare the antioxidant, cytotoxic, and antimicrobial effects of spindle leaves of L. () (oleaster) leaves.
Methods: Total phenolic content was measured using the Folin-Ciocalteu method, phenolic compound analysis by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) and antimicrobial effect by the minimum inhibition concentration (MIC) method.
MEF2C is a potential prognostic biomarker for osteosarcoma.
Medicine (Baltimore)
September 2025
Department of Orthopedic Surgery, Wenzhou TCM Hospital of Zhejiang Chinese Medical University, Wenzhou, China.
The purpose of this study was to investigate potential therapeutic targets for osteosarcoma (OS) and offer hints regarding genetic factors for OS treatment using a bioinformatics method. This study processed 3 OS datasets from the gene expression omnibus database using R software, screening for differentially expressed genes (DEGs). After enrichment analysis, based on expression quantitative trait loci data and the genome-wide association study data of OS, Mendelian randomization analysis was used to screen the genes closely related to OS disease, which intersect with DEGs to obtain co-expressed genes, validation datasets were employed to verify the results.
View Article and Find Full Text PDF