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Article Abstract
Objective: To investigate the mechanism of action of herb-partitioned moxibustion on CD from the perspective of autophagy and immunity.
Methods: The expression of microtubule-associated protein LC3II and SQSTM1/p62 in the colon tissues was detected by immunohistochemistry. Western blot was used to detect the expression of autophagic and immune-related proteins in the colon, such as LC3II, SQSTM1/p62, Beclin1, ATG16L1, NOD2, IRGM, IL-1, IL-17, and TNF-. mRNA levels of immune factors, such as IL-1, IL-17, and TNF-, and autophagy signaling molecules, such as PI3KC, AKT1, LKB1, and mTOR, were detected by RT-qPCR.
Results: Herb-partitioned moxibustion reduced the protein levels of ATG16L1, NOD2, IRGM, LC3II, and Beclin1 ( < 0.01) and both the protein and mRNA levels of IL-1, IL-17, and TNF- in CD rats ( < 0.01 or < 0.05), and it also increased the expression of SQSTM1/p62 protein ( < 0.01). The modulatory effects of herb-partitioned moxibustion on ATG16L1, NOD2, IRGM, LC3II, TNF-, and IL-17 protein and IL-1 protein and mRNA were better than those of mesalazine ( < 0.01 or < 0.05). Herb-partitioned moxibustion also reduced colon PI3KC, AKT1, and LKB1 mRNA expressions in CD rats ( < 0.01 or < 0.05) and increased mTOR protein expression ( < 0.05). And the modulatory effect of herb-partitioned moxibustion on AKT1 mRNA was better than that of mesalazine ( < 0.05).
Conclusion: Herb-partitioned moxibustion may inhibit excessively activated autophagy and modulate the expression of immune-related factors by regulating the LKB1-mTOR-PI3KC signal transduction networks, thereby alleviating intestinal inflammation in CD rats.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8816589 | PMC |
| http://dx.doi.org/10.1155/2022/3534874 | DOI Listing |
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