Differentiation of complex species isolated from invasive and non-invasive infections using gene size polymorphism.

Background And Purpose: Taxonomy of is controversial and has changed due to the investigation of the novel species. and are new members of the complex that are currently gaining both clinical and epidemiologic significance. This study aimed to report the prevalence of among the strains isolated from patients using hyphal wall protein 1 () gene size polymorphism.

Materials And Methods: In total, 235 yeasts confirmed as complex based on chromogenic media and internal transcribed spacers sequencing isolated from various clinical forms of invasive and non-invasive candidiasis mainly candidemia were re-identified using gene polymorphisms. The -polymerase chain reaction amplicons were re-confirmed by sequencing and BLAST analysis.

Results: Based on the gene size polymorphism, 223 strains were identified as (94.89%) from which 7 isolates produced two DNA fragments (850 and 941 bp). The (n=4, 1.7%), (n=1, 0.42%), and mix of and (n=7, 2.97%) were also identified.

Conclusion: It can be said that remains the most common species, while and are rarely found among the patient isolates. Due to limited information on the molecular epidemiology of this novel yeast, more studies using molecular methods are recommended.