Gene Amplification as a Mechanism of Yeast Adaptation to Nonsense Mutations in Release Factor Genes.

Protein synthesis (translation) is one of the fundamental processes occurring in the cells of living organisms. Translation can be divided into three key steps: initiation, elongation, and termination. In the yeast , there are two translation termination factors, eRF1 and eRF3. These factors are encoded by the and genes, which are essential; deletion of any of them leads to the death of yeast cells. However, viable strains with nonsense mutations in both the and genes were previously obtained in several groups. The survival of such mutants clearly involves feedback control of premature stop codon readthrough; however, the exact molecular basis of such feedback control remain unclear. To investigate the genetic factors supporting the viability of these and nonsense mutants, we performed whole-genome sequencing of strains carrying mutant and alleles; while no common SNPs or indels were found in these genomes, we discovered a systematic increase in the copy number of the plasmids carrying mutant and alleles. We used the qPCR method which confirmed the differences in the relative number of and gene copies between strains carrying wild-type or mutant alleles of and genes. Moreover, we compare the number of copies of the and genes in strains carrying different nonsense mutant variants of these genes as a single chromosomal copy. qPCR results indicate that the number of mutant gene copies is increased compared to the wild-type control. In case of several alleles, this was due to a disomy of the entire chromosome II, while for the mutation we observed a local duplication of a segment of chromosome IV containing the gene. Taken together, our results indicate that gene amplification is a common mechanism of adaptation to nonsense mutations in release factor genes in yeast.