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This article discusses the rationale for inclusion of flow cytometry (FCM) in the diagnostic investigation and evaluation of cytopenias of uncertain origin and suspected myelodysplastic syndromes (MDS) by the European LeukemiaNet international MDS Flow Working Group (ELN iMDS Flow WG). The WHO 2016 classification recognizes that FCM contributes to the diagnosis of MDS and may be useful for prognostication, prediction, and evaluation of response to therapy and follow-up of MDS patients.
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http://dx.doi.org/10.1002/cyto.b.22044 | DOI Listing |
Odontology
September 2025
Department of Biomaterials, Hamidiye Institute of Health Sciences, University of Health Sciences Turkey, Istanbul, Turkey.
This study evaluates the cytotoxicity, apoptosis, and expression of stress-related genes (TP53 and NF-κB) in response to gingiva-colored indirect composite resins used for veneering tooth or implant-supported prostheses or characterization of denture bases. A total of 120 disc-shaped specimens (2 mm thick, 10 mm diameter) gingiva-colored indirect composite resin specimens (Group A: Anaxgum-Anaxdent, Group B: Crealing Paste Gum-Bredent, Group G: Gradia Gum-GC, Group N: SR Nexco GUM-Ivoclar Vivadent) were prepared and divided into four groups (n = 10 per group). Surface wettability was assessed using water contact angle (WCA) measurements.
View Article and Find Full Text PDFNat Microbiol
September 2025
School of Oceanography, University of Washington, Seattle, WA, USA.
The cyanobacterium Prochlorococcus is Earth's most abundant photosynthetic organism and crucial to oceanic ecosystems. However, its sensitivity to a changing climate remains unclear. Here we analysed decade-long field measurements using continuous-flow cytometry from our SeaFlow instrument, collecting per-cell chlorophyll fluorescence and size data for ~800 billion phytoplankton cells across the tropical and subtropical Pacific Ocean to quantify the temperature dependence of cell division.
View Article and Find Full Text PDFNat Cell Biol
September 2025
Department of Biochemistry, University of Oxford, Oxford, UK.
In mammals, chromosome-wide regulatory mechanisms ensure a balance of X-linked gene dosage between males (XY) and females (XX). In female cells, expression of genes from one of the two X chromosomes is curtailed, with selective accumulation of Xist-RNA, Xist-associated proteins, specific histone modifications (for example, H3K27me3) and Barr body formation observed throughout interphase. Here we show, using chromosome flow-sorting, that during mitosis, Xist-associated proteins dissociate from inactive X (Xi) chromosomes, while high levels of H3K27me3 and increased compaction of the Xi relative to active X (Xa), are retained.
View Article and Find Full Text PDFBr J Haematol
September 2025
Department of Laboratory Medicine and Pathology, University of Washington, Seattle, Washington, USA.
Zhonghua Bing Li Xue Za Zhi
September 2025
Department of Hematology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
To investigate the clinicopathological features, diagnosis, and prognosis of aggressive natural killer-cell leukemia (ANKL). A retrospective analysis was conducted on 27 ANKL patients treated at the First Affiliated Hospital of Nanjing Medical University from 2014 to 2024. Their clinical data, histomorphology, and immunophenotype were reviewed.
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