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Article Abstract

An integrative study on some species of was carried out. Two species of gall midges from Italy, sp. nov. and sp. nov. (Diptera: Cecidomyiidae), causing flower galls respectively on and (Lamiaceae), are described and illustrated. The characteristics of each developmental stage and induced galls are described, which allowed the discrimination of these new species in the complex of developing on Lamiaceae plants. Molecular data based on sequencing both nuclear (ITS2 and 28S-D2) and mitochondrial (COI) genes are also provided in support of this discrimination. Phylogeny based on nuclear markers is consistent with the new species, whereas COI phylogeny suggests introgression occurring between the two species. However, these species can also be easily identified using a morphological approach. Phenology of host plants and gall midges are described, and some peculiar characteristics allow the complete and confident discrimination and revision of the treated species. Gall-associated fungi were identified as spp., and spp.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8619354PMC
http://dx.doi.org/10.3390/insects12110958DOI Listing

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