Screening and Identification of From Rumen Fluid of an Adult Female Sika Deer as a Potential Probiotic for Feed Additives.

Lactic acid bacteria (LAB) are the main microorganisms used as probiotics against gastrointestinal inflammation. The objective of this study was to evaluate the potential probiotic characteristics (antimicrobial activity, artificial gastrointestinal model resistance, cell surface hydrophobicity, and autoaggregation ability) and safety characteristics (hemolytic activity, antimicrobial resistance, and safety) of LAB isolated from the rumen fluid of an adult female sika deer. Two isolated strains identified as and showed good antimicrobial activity against enteropathogenic (ATCC25922), (ATCC14028), and (ATCC25923). In addition, exhibited higher artificial gastrointestinal model resistance, cell surface hydrophobicity and autoaggregation ability than . Therefore, regarding safety characteristics, only was evaluated. Upon assessment of safety, was negative for hemolytic activity and susceptible to penicillin G and cefamandole. Furthermore, an safety assessment showed that high-dose (10 CFU/mL) supplementation not only had no adverse effects on body weight gain, feed intake, and organ coefficients of treated mice but also played a key role in promoting the immune system maturation of treated mice. This research revealed that possesses desirable probiotic characteristics and could be used as a potential probiotic feed additive to improve sika deer health.