Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The cell cycle is a complex sequence of events by which cells grow and divide mitotically or meiotically. Mitosis results in the generation of two identical daughter cells, while meiosis generates gametes as a prerequisite for sexual reproduction. To study the localization and dynamics of proteins involved in the regulation and proceeding of the cell cycle, life cell imaging of proteins fused to fluorescent tags can be performed. However, in some cases this approach cannot be applied, e.g., due to low fluorescence intensity, fast bleaching, or degradation of recombinant proteins by the proteasome pathway. Instead, immunolabeling with protein-specific antibodies offers a useful approach for the analysis of intact cells. Alternatively, immunolabeling can also be applied to isolated and/or flow-sorted nuclei of particular cell cycle stages (G1, S, and G2) or of different endopolyploidy levels. The following chapter will detail indirect immunolabeling protocols to analyze the subcellular localization and distribution of cell cycle-specific proteins in Arabidopsis thaliana.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-0716-1744-1_2 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A Bird's-Eye View of the Wings Apart-Like Protein in Cell Biology and Implications for Disease and Therapeutics.
OMICS
September 2025
Centre for Integrative Omics Data Science (CIODS), Yenepoya (Deemed to be University), Mangalore, India.
Wings apart-like protein (WAPL) has emerged as a key player in maintaining genome integrity through its regulation of cohesin dynamics, which govern chromatin architecture and gene expression. WAPL mainly acts as a cohesin release factor and ensures proper chromosomal segregation during mitosis by promoting sister chromatid resolution. Owing to its prominent role in cell biology, WAPL dysregulation can cause genomic instability and disrupt chromosomal cohesion, leading to diseases such as cancer.
View Article and Find Full Text PDFTranscriptional Dynamics of Tomato Plants Under Combined Heat and Salt Stress.
Physiol Plant
September 2025
Centre of Molecular and Environmental Biology (CBMA), Department of Biology, School of Sciences of the University of Minho, Braga, Portugal.
The Mediterranean Basin, a hotspot for tomato production, is one of the most vulnerable areas to climate change, where rising temperatures and increasing soil and water salinization represent major threats to agricultural sustainability. Thus, to understand the molecular mechanisms behind plant responses to this stress combination, an RNA-Seq analysis was conducted on roots and shoots of tomato plants exposed to salt (100 mM NaCl) and/or heat (42°C, 4 h each day) stress for 21 days. The analysis identified over 8000 differentially expressed genes (DEGs) under combined stress conditions, with 1716 DEGs in roots and 2665 in shoots being exclusively modulated in response to this specific stress condition.
View Article and Find Full Text PDFPreoperative embolization improves local control and modulates gene expression in atypical WHO grade 2 meningioma.
Neuro Oncol
September 2025
Department of Neurological Surgery, University of California, San Francisco, San Francisco, CA, USA.
Background: Preoperative embolization is hypothesized to reduce blood loss and operative time for meningioma resection, but the impact of preoperative embolization on long-term oncological outcomes and molecular features of meningiomas is incompletely understood. Here we investigate how preoperative embolization influences perioperative and long-term outcomes and molecular features of atypical WHO grade 2 meningiomas.
Methods: Patients who underwent resection of WHO grade 2 meningiomas from 1997 to 2021 were retrospectively identified from an institutional database.
APC/C-CDH1 suppresses AROS-mediated protection against DNA damage-induced senescence by ubiquitination.
BMB Rep
September 2025
Department of Molecular Biology, Dankook University, Cheonan 31116, Korea.
Anaphase-promoting complex/cyclosome (APC/C) regulates the cell cycle by destruction of target proteins ubiquitination. However, understanding the control of APC/C has remained elusive. We identify APC2, the catalytic core subunit of APC/C, as a binding partner of active regulator of SIRT1 (AROS).
View Article and Find Full Text PDFLuteolin Enhances Anticancer Effects of PX-478 during Hypoxic Response in Metastatic Breast Cancer Cells.
Anticancer Agents Med Chem
September 2025
Molecular Biology and Genetics Department, Faculty of Arts and Science, Burdur Mehmet Akif Ersoy University, Burdur, 15100, Turkey.
Introduction: The presence of severe hypoxic stress can drive tumor growth, angiogenesis, and metastatic characteristics via up-regulated hypoxia-inducible factor 1-alpha (HIF-1α). Hence, targeting HIF-1α is considered a promising strategy, as increased HIF-1α activity is a key factor in the aggressive phenotype of malignancies. In this study, we aimed to investigate the anti-cancer effects of several flavonoids, both single and in combination with PX-478, in breast cancer cell lines.
View Article and Find Full Text PDF