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PCR-based next-generation West Nile virus sequencing protocols. | LitMetric
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PCR-based next-generation West Nile virus sequencing protocols.

Styliani Pappa , Serafeim C Chaintoutis , Chrysostomos I Dovas , Anna Papa

Mol Cell Probes

National Reference Centre for Arboviruses, Department of Microbiology, Medical School, Faculty of Health Sciences, Aristotle University of Thessaloniki, Greece. Electronic address:

Published: December 2021

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Article Abstract

The epidemiology of West Nile virus (WNV) is unpredictable and changing. Availability of whole genome sequences enables the detailed molecular epidemiology studies and the evaluation and design of diagnostic tools. In the present study we provide two PCR-based protocols which can be applied directly on biological samples from hosts infected by WNV strains belonging to lineage 1 or lineage 2. It was shown that the protocols worked successfully even on samples with relatively low viral load.

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 Source
http://dx.doi.org/10.1016/j.mcp.2021.101774DOI Listing

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