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http://dx.doi.org/10.2217/nnm-2021-0035 | DOI Listing |
RSC Chem Biol
August 2025
Innovation Center, Research Division Kyowa Kirin Co., Ltd. 3-6-6 Asahi, Machida Tokyo 194-8533 Japan
The poly(A) tail plays a crucial role in mRNA stability and translation efficiency. Chemical modification of the poly(A) tail is a promising approach for stabilizing mRNA against deadenylation. In this study, we investigated the effect of poly(A) chemical modifications using phosphorothioate (PS), 2'-fluoro (2'-F), 2'--methyl (2'-OMe), and 2'--methoxyethyl (2'-MOE) modifications.
View Article and Find Full Text PDFMol Ther Nucleic Acids
September 2025
Department of Advanced Nanomedical Engineering, Medical Research Laboratory, Institute of Integrated Research, Institute of Science Tokyo, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
Vaccine
August 2025
Department of Advanced Nanomedical Engineering, Medical Research Laboratory, Institute of Integrated Research, Institute of Science Tokyo, 1-5-45 Yushima Bunkyo-ku Tokyo, 113-8510, Japan; Innovation Center of NanoMedicine (iCONM), Kawasaki Institute of Industrial Promotion, 3-25-14 Tonomachi Kawasak
Retinochoroidal neovascularization (NV), involved in macular degeneration, diabetic retinopathy, and other ocular diseases, causes vision impairment and blindness. Current treatments rely on repeated intraocular injections of anti-angiogenic drugs, which are burdensome for patients and clinicians, and some patients fail to respond to the treatments. This study investigates the potential of mRNA vaccination to mitigate NV and treat ocular pathologies.
View Article and Find Full Text PDFNanoscale Horiz
August 2025
Department of Chemistry, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Florence, Italy.
The integration of gold nanoparticles (AuNPs) with lipid bilayers gives rise to powerful synergistic effects arising from nanoscale interactions. Precise control over these interactions enables the rational design of hybrid AuNP-lipid membrane multifunctional composites, unlocking advanced analytical tools and cutting-edge biomedical applications. From a materials design standpoint, functionalizing AuNPs with lipid membranes reduces cytotoxicity and enhances stability in complex biological environments.
View Article and Find Full Text PDFJ Control Release
August 2025
Innovation Center of NanoMedicine (iCONM), Kawasaki Institute of Industrial Promotion, 3-25-14 Tonomachi, Kawasaki-ku, Kawasaki 210-0821, Japan. Electronic address:
Polycation-based mRNA delivery systems have an issue with mRNA integrity in the physiological milieu, particularly in blood compartments, despite their vast potential in mRNA therapeutics. Without comprehensive mechanistic analyses, design concepts of polyplexes for in vivo use remain unclear. Herein, we systematically assessed several potential design parameters of polyplex stabilization and provided mechanistic insight into the processes of mRNA degradation loaded in polyplexes, focusing on RNase attack, a process believed to be the leading cause of loss of mRNA integrity loaded into polyplexes.
View Article and Find Full Text PDF