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Human gait analysis is often conducted in clinical and basic research, but many common approaches (e.g., three-dimensional motion capture, wearables) are expensive, immobile, data-limited, and require expertise. Recent advances in video-based pose estimation suggest potential for gait analysis using two-dimensional video collected from readily accessible devices (e.g., smartphones). To date, several studies have extracted features of human gait using markerless pose estimation. However, we currently lack evaluation of video-based approaches using a dataset of human gait for a wide range of gait parameters on a stride-by-stride basis and a workflow for performing gait analysis from video. Here, we compared spatiotemporal and sagittal kinematic gait parameters measured with OpenPose (open-source video-based human pose estimation) against simultaneously recorded three-dimensional motion capture from overground walking of healthy adults. When assessing all individual steps in the walking bouts, we observed mean absolute errors between motion capture and OpenPose of 0.02 s for temporal gait parameters (i.e., step time, stance time, swing time and double support time) and 0.049 m for step lengths. Accuracy improved when spatiotemporal gait parameters were calculated as individual participant mean values: mean absolute error was 0.01 s for temporal gait parameters and 0.018 m for step lengths. The greatest difference in gait speed between motion capture and OpenPose was less than 0.10 m s-1. Mean absolute error of sagittal plane hip, knee and ankle angles between motion capture and OpenPose were 4.0°, 5.6° and 7.4°. Our analysis workflow is freely available, involves minimal user input, and does not require prior gait analysis expertise. Finally, we offer suggestions and considerations for future applications of pose estimation for human gait analysis.
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http://dx.doi.org/10.1371/journal.pcbi.1008935 | DOI Listing |
Spine (Phila Pa 1976)
October 2025
Niigata Spine Surgery Center, Kameda Daiichi Hospital, Niigata, Japan.
Study Design: Prospective cohort study.
Objective: To investigate longitudinal changes in physical functional status after long corrective fusion in patients with adult spinal deformity (ASD) during 2 years of follow-up.
Background: In ASD surgery, reports assessing physical functional status in long-term observations for more than a year are lacking.
Ann Neurol
September 2025
Spinal Cord Injury Center, Balgrist University Hospital, University of Zurich, Zurich, Switzerland.
Objective: Impaired ability to induce stepping after incomplete spinal cord injury (SCI) can limit the efficacy of locomotor training, often leaving patients wheelchair-bound. The cuneiform nucleus (CNF), a key mesencephalic locomotor control center, modulates the activity of spinal locomotor centers via the reticulospinal tract. Even with severe corticospinal damage, the widely distributed reticulospinal fibers frequently cross the lesion, and lumbosacral spinal locomotor centers remain responsive.
View Article and Find Full Text PDFNEJM AI
September 2025
Department of Bioengineering, Stanford University, Stanford, CA.
Background: Assessing human movement is essential for diagnosing and monitoring movement-related conditions like neuromuscular disorders. Timed function tests (TFTs) are among the most widespread types of assessments due to their speed and simplicity, but they cannot capture disease-specific movement patterns. Conversely, biomechanical analysis can produce sensitive disease-specific biomarkers, but it is traditionally confined to laboratory settings.
View Article and Find Full Text PDFCureus
August 2025
Department of Orthopedic Surgery, Matsuda Orthopedic Clinic, Kumagaya, JPN.
Background: The effect of supplementation of essential amino acids (EAAs) in knee osteoarthritis (OA) remains unclear. This study aimed to evaluate whether supplementation with EAA improves pain, patient-reported outcome measures, gait function, and quadriceps muscle volume in patients undergoing conservative treatment for knee OA.
Methods: A retrospective cohort study was conducted on outpatients undergoing physical therapy from April 2024 to March 2025.
Temperature (Austin)
April 2025
Department of Health and Human Performance, Texas State University, San Marcos, TX, USA.
A heat tolerance test (HTT) can aid in return-to-play decision making following exertional heat stroke (EHS). The HTT uses rectal temperature (T, >38.5°C) and heart rate thresholds (HR; >150 bpm) to identify "heat intolerance.
View Article and Find Full Text PDF